Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 128506..129041 Replicon plasmid p87_1_193kb
Accession NZ_CP066458
Organism Enterococcus faecium strain Dallas 87_1

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag JCP85_RS14720 Protein ID WP_002305108.1
Coordinates 128506..128778 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag JCP85_RS14725 Protein ID WP_002303154.1
Coordinates 128778..129041 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP85_RS14690 123512..124699 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
JCP85_RS14695 124887..125834 + 948 WP_002303155.1 DNA-binding transcriptional regulator -
JCP85_RS14700 126131..126679 - 549 WP_002292681.1 hypothetical protein -
JCP85_RS14705 126680..127537 - 858 WP_002292680.1 AAA family ATPase -
JCP85_RS14710 127823..128110 - 288 WP_002313074.1 type II toxin-antitoxin system RelE/ParE family toxin -
JCP85_RS14715 128100..128429 - 330 WP_002292678.1 AbrB family transcriptional regulator -
JCP85_RS14720 128506..128778 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
JCP85_RS14725 128778..129041 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JCP85_RS14730 129147..129563 - 417 WP_002303153.1 hypothetical protein -
JCP85_RS14735 129569..130132 - 564 Protein_139 IS6 family transposase -
JCP85_RS14740 130212..131374 + 1163 WP_086956687.1 IS3 family transposase -
JCP85_RS14745 131846..132745 - 900 WP_002303235.1 aldose 1-epimerase family protein -
JCP85_RS14750 132766..133038 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..193594 193594


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T184448 WP_002305108.1 NZ_CP066458:c128778-128506 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T184448 NZ_CP087657:c2779531-2778209 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT184448 WP_002303154.1 NZ_CP066458:c129041-128778 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT184448 NZ_CP087657:c2779797-2779531 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References