Detailed information of TA system TA184442

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 46706..46911
• Replicon: plasmid p83_55kb
• Accession: NZ_CP066453
• Organism: Enterococcus faecium strain Dallas 83

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP60_RS14995
• Protein ID: WP_002295624.1
• Coordinates: 46706..46828 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	46810..46911 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP60_RS14955	41782..41985	-	204	WP_002300097.1	hypothetical protein	-
JCP60_RS14960	42145..42720	-	576	WP_002300096.1	recombinase family protein	-
JCP60_RS14965	42736..43341	-	606	WP_002300095.1	Fic family protein	-
JCP60_RS14970	43947..44126	-	180	WP_002305204.1	hypothetical protein	-
JCP60_RS14975	44507..45214	-	708	WP_002305202.1	hypothetical protein	-
JCP60_RS14980	45207..45644	-	438	WP_002287515.1	hypothetical protein	-
JCP60_RS14985	45649..45849	-	201	WP_002305199.1	hypothetical protein	-
JCP60_RS14990	46292..46552	-	261	WP_049055261.1	hypothetical protein	-
JCP60_RS14995	46706..46828	+	123	WP_002295624.1	putative holin-like toxin	Toxin
-	46810..46911	-	102	NuclAT_0	-	Antitoxin
JCP60_RS15000	46988..47245	-	258	WP_002305195.1	hypothetical protein	-
JCP60_RS15005	47267..47752	-	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP60_RS15010	47755..48654	-	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP60_RS15015	48670..49263	-	594	WP_002287520.1	transcriptional regulator	-
JCP60_RS15020	49337..49486	-	150	WP_002295632.1	hypothetical protein	-
JCP60_RS15025	49502..51706	-	2205	WP_025481008.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55497	55497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184442 WP_002295624.1 NZ_CP066453:46706-46828 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184442 NZ_CP087655:c3735-3421 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184442 NZ_CP066453:c46911-46810 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin