Detailed information of TA system TA184436

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 45473..45678
• Replicon: plasmid p71_2_57kb
• Accession: NZ_CP066445
• Organism: Enterococcus faecium strain Dallas 71_2

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP66_RS15400
• Protein ID: WP_002295624.1
• Coordinates: 45556..45678 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	45473..45574 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP66_RS15370	40678..42882	+	2205	WP_129974412.1	type IA DNA topoisomerase	-
JCP66_RS15375	42898..43047	+	150	WP_002295632.1	hypothetical protein	-
JCP66_RS15380	43121..43714	+	594	WP_002287520.1	transcriptional regulator	-
JCP66_RS15385	43730..44629	+	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP66_RS15390	44632..45117	+	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP66_RS15395	45139..45396	+	258	WP_002305195.1	hypothetical protein	-
-	45473..45574	+	102	NuclAT_0	-	Antitoxin
JCP66_RS15400	45556..45678	-	123	WP_002295624.1	putative holin-like toxin	Toxin
JCP66_RS15405	45832..46092	+	261	WP_049055261.1	hypothetical protein	-
JCP66_RS15410	46535..46735	+	201	WP_002305199.1	hypothetical protein	-
JCP66_RS15415	46740..47177	+	438	WP_002287515.1	hypothetical protein	-
JCP66_RS15420	47170..47877	+	708	WP_002305202.1	hypothetical protein	-
JCP66_RS15425	48258..48437	+	180	WP_002305204.1	hypothetical protein	-
JCP66_RS15430	48619..49914	-	1296	WP_002297218.1	ISL3-like element ISEfa11 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57029	57029
	-	flank	IS/Tn	-	-	48619..49914	1295

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184436 WP_002295624.1 NZ_CP066445:c45678-45556 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184436 NZ_CP087651:c5388009-5387626 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184436 NZ_CP066445:45473-45574 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin