Detailed information of TA system TA184429

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 52677..52882
• Replicon: plasmid p66_57kb
• Accession: NZ_CP066437
• Organism: Enterococcus faecium strain Dallas 66

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP89_RS15510
• Protein ID: WP_002295624.1
• Coordinates: 52760..52882 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	52677..52778 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP89_RS15480	47882..50086	+	2205	WP_129974412.1	type IA DNA topoisomerase	-
JCP89_RS15485	50102..50251	+	150	WP_002295632.1	hypothetical protein	-
JCP89_RS15490	50325..50918	+	594	WP_002287520.1	transcriptional regulator	-
JCP89_RS15495	50934..51833	+	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP89_RS15500	51836..52321	+	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP89_RS15505	52343..52600	+	258	WP_002305195.1	hypothetical protein	-
-	52677..52778	+	102	NuclAT_0	-	Antitoxin
JCP89_RS15510	52760..52882	-	123	WP_002295624.1	putative holin-like toxin	Toxin
JCP89_RS15515	53036..53296	+	261	WP_049055261.1	hypothetical protein	-
JCP89_RS15520	53739..53939	+	201	WP_002305199.1	hypothetical protein	-
JCP89_RS15525	53944..54381	+	438	WP_002287515.1	hypothetical protein	-
JCP89_RS15530	54374..55081	+	708	WP_002305202.1	hypothetical protein	-
JCP89_RS15535	55462..55641	+	180	WP_002305204.1	hypothetical protein	-
JCP89_RS15540	56247..56852	+	606	WP_002300095.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57014	57014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184429 WP_002295624.1 NZ_CP066437:c52882-52760 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184429 NZ_CP087651:878651-879058 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184429 NZ_CP066437:52677-52778 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin