Detailed information of TA system TA184424

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 16339..16544
• Replicon: plasmid p57_57kb
• Accession: NZ_CP066430
• Organism: Enterococcus faecium strain Dallas 57

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP98_RS15255
• Protein ID: WP_002295624.1
• Coordinates: 16422..16544 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	16339..16440 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP98_RS15225	11544..13748	+	2205	WP_129974412.1	type IA DNA topoisomerase	-
JCP98_RS15230	13764..13913	+	150	WP_002295632.1	hypothetical protein	-
JCP98_RS15235	13987..14580	+	594	WP_002287520.1	transcriptional regulator	-
JCP98_RS15240	14596..15495	+	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP98_RS15245	15498..15983	+	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP98_RS15250	16005..16262	+	258	WP_002305195.1	hypothetical protein	-
-	16339..16440	+	102	NuclAT_0	-	Antitoxin
JCP98_RS15255	16422..16544	-	123	WP_002295624.1	putative holin-like toxin	Toxin
JCP98_RS15260	16698..16958	+	261	WP_049055261.1	hypothetical protein	-
JCP98_RS15265	17401..17601	+	201	WP_002305199.1	hypothetical protein	-
JCP98_RS15270	17606..18043	+	438	WP_002287515.1	hypothetical protein	-
JCP98_RS15275	18036..18743	+	708	WP_002305202.1	hypothetical protein	-
JCP98_RS15280	19124..19303	+	180	WP_002305204.1	hypothetical protein	-
JCP98_RS15285	19485..20780	-	1296	WP_002297218.1	ISL3-like element ISEfa11 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57029	57029
	-	flank	IS/Tn	-	-	19485..20780	1295

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184424 WP_002295624.1 NZ_CP066430:c16544-16422 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184424 NZ_CP087647:181564-181713 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184424 NZ_CP066430:16339-16440 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin