Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 135952..136487 Replicon plasmid p55_202kb
Accession NZ_CP066422
Organism Enterococcus faecium strain Dallas 55

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag JCP69_RS14615 Protein ID WP_002305108.1
Coordinates 135952..136224 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag JCP69_RS14620 Protein ID WP_002303154.1
Coordinates 136224..136487 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP69_RS14585 130958..132145 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
JCP69_RS14590 132333..133280 + 948 WP_002303155.1 DNA-binding transcriptional regulator -
JCP69_RS14595 133577..134125 - 549 WP_002292681.1 hypothetical protein -
JCP69_RS14600 134126..134983 - 858 WP_002292680.1 AAA family ATPase -
JCP69_RS14605 135269..135556 - 288 WP_002313074.1 type II toxin-antitoxin system RelE/ParE family toxin -
JCP69_RS14610 135546..135875 - 330 WP_002292678.1 AbrB family transcriptional regulator -
JCP69_RS14615 135952..136224 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
JCP69_RS14620 136224..136487 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JCP69_RS14625 136593..137009 - 417 WP_002303153.1 hypothetical protein -
JCP69_RS14630 137015..137578 - 564 Protein_147 IS6 family transposase -
JCP69_RS14635 137658..138820 + 1163 WP_086956687.1 IS3 family transposase -
JCP69_RS14640 139292..140191 - 900 WP_002303235.1 aldose 1-epimerase family protein -
JCP69_RS14645 140212..140484 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..202167 202167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T184419 WP_002305108.1 NZ_CP066422:c136224-135952 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T184419 NZ_CP087645:c4920242-4919958 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT184419 WP_002303154.1 NZ_CP066422:c136487-136224 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT184419 NZ_CP087645:c4920474-4920232 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References