Detailed information of TA system TA184413

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 17358..17563
• Replicon: plasmid p53_1_55kb
• Accession: NZ_CP066416
• Organism: Enterococcus faecium strain Dallas 53_1

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP73_RS14210
• Protein ID: WP_002295624.1
• Coordinates: 17358..17480 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	17462..17563 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP73_RS14170	12434..12637	-	204	WP_002300097.1	hypothetical protein	-
JCP73_RS14175	12797..13372	-	576	WP_002300096.1	recombinase family protein	-
JCP73_RS14180	13388..13993	-	606	WP_002300095.1	Fic family protein	-
JCP73_RS14185	14599..14778	-	180	WP_002305204.1	hypothetical protein	-
JCP73_RS14190	15159..15866	-	708	WP_002305202.1	hypothetical protein	-
JCP73_RS14195	15859..16296	-	438	WP_002287515.1	hypothetical protein	-
JCP73_RS14200	16301..16501	-	201	WP_002305199.1	hypothetical protein	-
JCP73_RS14205	16944..17204	-	261	WP_049055261.1	hypothetical protein	-
JCP73_RS14210	17358..17480	+	123	WP_002295624.1	putative holin-like toxin	Toxin
-	17462..17563	-	102	NuclAT_0	-	Antitoxin
JCP73_RS14215	17640..17897	-	258	WP_002305195.1	hypothetical protein	-
JCP73_RS14220	17919..18404	-	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP73_RS14225	18407..19306	-	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP73_RS14230	19322..19915	-	594	WP_002287520.1	transcriptional regulator	-
JCP73_RS14235	19989..20138	-	150	WP_002295632.1	hypothetical protein	-
JCP73_RS14240	20154..22358	-	2205	WP_025481008.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55498	55498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184413 WP_002295624.1 NZ_CP066416:17358-17480 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184413 NZ_CP087645:744965-745450 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184413 NZ_CP066416:c17563-17462 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin