Detailed information of TA system TA184407

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 16329..16534
• Replicon: plasmid p53_2_55kb
• Accession: NZ_CP066411
• Organism: Enterococcus faecium strain Dallas 53_2

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E3USR6
• Locus tag: JCP94_RS15210
• Protein ID: WP_002295624.1
• Coordinates: 16412..16534 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	16329..16430 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCP94_RS15180	11534..13738	+	2205	WP_025481008.1	type IA DNA topoisomerase	-
JCP94_RS15185	13754..13903	+	150	WP_002295632.1	hypothetical protein	-
JCP94_RS15190	13977..14570	+	594	WP_002287520.1	transcriptional regulator	-
JCP94_RS15195	14586..15485	+	900	WP_002305191.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JCP94_RS15200	15488..15973	+	486	WP_002295626.1	single-stranded DNA-binding protein	-
JCP94_RS15205	15995..16252	+	258	WP_002305195.1	hypothetical protein	-
-	16329..16430	+	102	NuclAT_0	-	Antitoxin
JCP94_RS15210	16412..16534	-	123	WP_002295624.1	putative holin-like toxin	Toxin
JCP94_RS15215	16688..16948	+	261	WP_049055261.1	hypothetical protein	-
JCP94_RS15220	17391..17591	+	201	WP_002305199.1	hypothetical protein	-
JCP94_RS15225	17596..18033	+	438	WP_002287515.1	hypothetical protein	-
JCP94_RS15230	18026..18733	+	708	WP_002305202.1	hypothetical protein	-
JCP94_RS15235	19114..19293	+	180	WP_002305204.1	hypothetical protein	-
JCP94_RS15240	19899..20504	+	606	WP_002300095.1	Fic family protein	-
JCP94_RS15245	20520..21095	+	576	WP_002300096.1	recombinase family protein	-
JCP94_RS15250	21255..21458	+	204	WP_002300097.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55497	55497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (7-40)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4431.42 Da
• Isoelectric Point: 10.3686

>T184407 WP_002295624.1 NZ_CP066411:c16534-16412 [Enterococcus faecium]
MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK

Nucleotide

• Length: 123 bp

>T184407 NZ_CP087641:c41043-40732 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Nucleotide

• Length: 102 bp

>AT184407 NZ_CP066411:16329-16430 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Toxin

• AlphaFold DB: E3USR6

Antitoxin