Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 244354..244889 Replicon plasmid p16_1_257kb
Accession NZ_CP066382
Organism Enterococcus faecium strain Dallas 16_1

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag JCP87_RS14850 Protein ID WP_002314366.1
Coordinates 244354..244626 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag JCP87_RS14855 Protein ID WP_002297422.1
Coordinates 244626..244889 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP87_RS14810 239460..239909 - 450 WP_010729386.1 TlpA family protein disulfide reductase -
JCP87_RS14815 240065..240607 - 543 WP_002298088.1 hypothetical protein -
JCP87_RS14820 240882..241525 + 644 Protein_271 transposase -
JCP87_RS14825 241601..242281 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
JCP87_RS14830 242340..242528 - 189 Protein_273 hypothetical protein -
JCP87_RS14835 242529..243386 - 858 WP_002292680.1 AAA family ATPase -
JCP87_RS14840 243672..243958 - 287 Protein_275 type II toxin-antitoxin system RelE/ParE family toxin -
JCP87_RS14845 243948..244277 - 330 WP_002292678.1 AbrB family transcriptional regulator -
JCP87_RS14850 244354..244626 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
JCP87_RS14855 244626..244889 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JCP87_RS14860 245123..246031 + 909 Protein_279 IS982 family transposase -
JCP87_RS14865 246034..247209 - 1176 WP_002336205.1 MFS transporter -
JCP87_RS14870 247212..249461 - 2250 WP_106017163.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..257742 257742
- inside IScluster/Tn - - 232791..255490 22699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T184392 WP_002314366.1 NZ_CP066382:c244626-244354 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T184392 NZ_CP087637:1031-1345 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT184392 WP_002297422.1 NZ_CP066382:c244889-244626 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT184392 NZ_CP087637:765-1043 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References