Detailed information of TA system TA184375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 17192..17715
• Replicon: plasmid pSE1
• Accession: NZ_CP066375
• Organism: Staphylococcus epidermidis strain PH1-28

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: ICN77_RS00515
• Protein ID: WP_145353822.1
• Coordinates: 17449..17715 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C5QR06
• Locus tag: ICN77_RS00510
• Protein ID: WP_002441972.1
• Coordinates: 17192..17449 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICN77_RS00475	12592..13413	-	822	Protein_12	hypothetical protein	-
ICN77_RS00480	13425..13808	-	384	WP_103463593.1	hypothetical protein	-
ICN77_RS00485	13810..14088	-	279	WP_103463592.1	hypothetical protein	-
ICN77_RS00490	14936..15097	-	162	WP_198895615.1	hypothetical protein	-
ICN77_RS00495	15635..16309	-	675	WP_001105987.1	IS6-like element IS257 family transposase	-
ICN77_RS00500	16456..16635	+	180	WP_198895616.1	CsbD family protein	-
ICN77_RS00505	16668..17027	-	360	WP_103463604.1	YolD-like family protein	-
ICN77_RS00510	17192..17449	+	258	WP_002441972.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ICN77_RS00515	17449..17715	+	267	WP_145353822.1	Txe/YoeB family addiction module toxin	Toxin
ICN77_RS00520	17732..17902	+	171	WP_016898857.1	hypothetical protein	-
ICN77_RS00525	18248..18520	+	273	WP_198895617.1	hypothetical protein	-
ICN77_RS00530	18818..19366	+	549	WP_064657848.1	recombinase family protein	-
ICN77_RS00535	19929..20195	-	267	WP_145353820.1	Txe/YoeB family addiction module toxin	-
ICN77_RS00540	20188..20442	-	255	WP_002452575.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ICN77_RS00545	20566..20739	+	174	WP_145353838.1	plasmid recombination protein	-
ICN77_RS00550	21393..21721	-	329	Protein_27	replication protein	-
ICN77_RS00555	22103..22273	+	171	WP_002456306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51568	51568
	-	flank	IS/Tn	-	-	15635..16309	674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10691.35 Da
• Isoelectric Point: 10.3481

>T184375 WP_145353822.1 NZ_CP066375:17449-17715 [Staphylococcus epidermidis]
MAKYKINIKNSAKTDLKKIKRSHLQEQFLKIVEILKYNPYQQSQSFEKLHPKHLGRYSRRINHQHRVVYTIDEENKEVLI
LAAWSHYE

Nucleotide

• Length: 267 bp

>T184375 NZ_CP087631:c102929-102486 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10041.10 Da
• Isoelectric Point: 4.5610

>AT184375 WP_002441972.1 NZ_CP066375:17192-17449 [Staphylococcus epidermidis]
MKSKTPTEARKDFYQLLKRVNNDHEPVYIEGKYDESNAVIIGMEDWRSINETIYLEATGTMDKVREREHDNSGYTNIDDI
DWARL

Nucleotide

• Length: 258 bp

>AT184375 NZ_CP087631:c103156-102926 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B5RUG0