Detailed information of TA system TA184360

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42909..43162
• Replicon: plasmid unnamed1
• Accession: NZ_CP066367
• Organism: Escherichia coli strain AR Bank #0346

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FNX50_RS22935
• Protein ID: WP_001312851.1
• Coordinates: 43013..43162 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	42909..42968 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNX50_RS22910 (39636)	39636..40382	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
FNX50_RS22915 (40437)	40437..40997	+	561	WP_000139328.1	fertility inhibition protein FinO	-
FNX50_RS22920 (41129)	41129..41329	+	201	WP_015059022.1	hypothetical protein	-
FNX50_RS22925 (41715)	41715..42314	+	600	WP_032083981.1	PIN domain-containing protein	-
FNX50_RS22930 (42376)	42376..42708	+	333	WP_152916585.1	hypothetical protein	-
- (42909)	42909..42968	-	60	NuclAT_1	-	Antitoxin
- (42909)	42909..42968	-	60	NuclAT_1	-	Antitoxin
- (42909)	42909..42968	-	60	NuclAT_1	-	Antitoxin
- (42909)	42909..42968	-	60	NuclAT_1	-	Antitoxin
FNX50_RS22935 (43013)	43013..43162	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
FNX50_RS22940 (43446)	43446..43694	+	249	WP_000083837.1	replication regulatory protein RepA	-
FNX50_RS24220 (43939)	43939..44013	+	75	WP_001375168.1	RepA leader peptide Tap	-
FNX50_RS22950 (44006)	44006..44863	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
FNX50_RS22955 (45802)	45802..46455	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
FNX50_RS22960 (46548)	46548..46805	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
FNX50_RS22965 (46738)	46738..47139	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
FNX50_RS22970 (47388)	47388..47803	+	416	Protein_58	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaCTX-M-55	-	1..101116	101116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184360 WP_001312851.1 NZ_CP066367:43013-43162 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184360 NZ_CP087626:c8087-7773 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT184360 NZ_CP066367:c42968-42909 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin