Detailed information of TA system TA184357

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3431..3700
• Replicon: plasmid unnamed1
• Accession: NZ_CP066367
• Organism: Escherichia coli strain AR Bank #0346

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FNX50_RS22705
• Protein ID: WP_001372321.1
• Coordinates: 3575..3700 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	3431..3496 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNX50_RS22685	20..2043	+	2024	Protein_0	ParB/RepB/Spo0J family partition protein	-
FNX50_RS22690	2112..2546	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FNX50_RS22695	2543..3305	+	763	Protein_2	plasmid SOS inhibition protein A	-
-	3274..3498	+	225	NuclAT_0	-	-
-	3274..3498	+	225	NuclAT_0	-	-
-	3274..3498	+	225	NuclAT_0	-	-
-	3274..3498	+	225	NuclAT_0	-	-
FNX50_RS22700	3283..3462	-	180	WP_001309233.1	hypothetical protein	-
-	3431..3496	-	66	-	-	Antitoxin
FNX50_RS24020	3484..3633	+	150	Protein_4	plasmid maintenance protein Mok	-
FNX50_RS22705	3575..3700	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
FNX50_RS22710	4019..4315	-	297	Protein_6	hypothetical protein	-
FNX50_RS22715	4615..4911	+	297	WP_001272251.1	hypothetical protein	-
FNX50_RS22720	5022..5843	+	822	WP_001234445.1	DUF932 domain-containing protein	-
FNX50_RS22725	6140..6787	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
FNX50_RS22730	7064..7447	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
FNX50_RS22735	7638..8324	+	687	WP_015059009.1	PAS domain-containing protein	-
FNX50_RS22740	8418..8645	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaCTX-M-55	-	1..101116	101116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T184357 WP_001372321.1 NZ_CP066367:3575-3700 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T184357 NZ_CP087624:c84563-84093 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT184357 NZ_CP066367:c3496-3431 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin