Detailed information of TA system TA184355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3915707..3916401
• Replicon: chromosome
• Accession: NZ_CP066366
• Organism: Escherichia coli strain AR Bank #0346

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FNX50_RS18940
• Protein ID: WP_001263489.1
• Coordinates: 3916003..3916401 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FNX50_RS18935
• Protein ID: WP_000554758.1
• Coordinates: 3915707..3916000 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNX50_RS18915 (3911339)	3911339..3911836	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
FNX50_RS18920 (3912060)	3912060..3913772	-	1713	Protein_3743	flagellar biosynthesis protein FlhA	-
FNX50_RS18925 (3913744)	3913744..3914529	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FNX50_RS18930 (3914600)	3914600..3915655	+	1056	WP_001226164.1	DNA polymerase IV	-
FNX50_RS18935 (3915707)	3915707..3916000	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FNX50_RS18940 (3916003)	3916003..3916401	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FNX50_RS18945 (3916411)	3916411..3916863	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FNX50_RS18950 (3917181)	3917181..3917387	+	207	Protein_3749	RtcB family protein	-
FNX50_RS18955 (3917383)	3917383..3917904	+	522	Protein_3750	peptide chain release factor H	-
FNX50_RS18960 (3917961)	3917961..3919418	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FNX50_RS18965 (3919679)	3919679..3920137	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (3920733)	3920733..3920813	+	81	NuclAT_11	-	-
- (3920733)	3920733..3920813	+	81	NuclAT_11	-	-
- (3920733)	3920733..3920813	+	81	NuclAT_11	-	-
- (3920733)	3920733..3920813	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T184355 WP_001263489.1 NZ_CP066366:3916003-3916401 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T184355 NZ_CP087624:19053-19469 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT184355 WP_000554758.1 NZ_CP066366:3915707-3916000 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT184355 NZ_CP087624:18826-19056 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3