Detailed information of TA system TA184320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1114054..1114672
• Replicon: chromosome
• Accession: NZ_CP066359
• Organism: Sinorhizobium meliloti strain AK76

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: JFX10_RS13015
• Protein ID: WP_010969011.1
• Coordinates: 1114376..1114672 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JFX10_RS13010
• Protein ID: WP_010969010.1
• Coordinates: 1114054..1114362 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX10_RS12965	1109133..1109453	-	321	WP_003527320.1	YnfA family protein	-
JFX10_RS12970	1109528..1109701	-	174	WP_010969006.1	hypothetical protein	-
JFX10_RS12975	1109715..1109888	-	174	WP_003527318.1	hypothetical protein	-
JFX10_RS12980	1110124..1110825	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
JFX10_RS12985	1111049..1111390	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
JFX10_RS12990	1111975..1112241	+	267	WP_003527312.1	sel1 repeat family protein	-
JFX10_RS12995	1112263..1113384	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
JFX10_RS13005	1113813..1114043	-	231	WP_026029805.1	hypothetical protein	-
JFX10_RS13010	1114054..1114362	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
JFX10_RS13015	1114376..1114672	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX10_RS13020	1114720..1115025	-	306	WP_003527305.1	hypothetical protein	-
JFX10_RS13025	1115353..1116390	+	1038	WP_017271047.1	porin	-
JFX10_RS13030	1116500..1117567	-	1068	WP_014526650.1	site-specific integrase	-
JFX10_RS13035	1117608..1118657	-	1050	WP_017271048.1	porin	-
JFX10_RS13040	1118871..1119359	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T184320 WP_010969011.1 NZ_CP066359:c1114672-1114376 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T184320 NZ_CP087612:40066-40353 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT184320 WP_010969010.1 NZ_CP066359:c1114362-1114054 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT184320 NZ_CP087612:39827-40066 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: Q92R61