Detailed information of TA system TA184278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 67726..68417
• Replicon: chromosome
• Accession: NZ_CP066310
• Organism: Azotobacter chroococcum strain HR1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GKQ51_RS00315
• Protein ID: WP_198866969.1
• Coordinates: 68130..68417 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0C4WIL7
• Locus tag: GKQ51_RS00310
• Protein ID: WP_039800694.1
• Coordinates: 67726..68127 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ51_RS00285	63131..64492	-	1362	WP_198866188.1	IS701 family transposase	-
GKQ51_RS00290	64684..64989	+	306	WP_144411487.1	hypothetical protein	-
GKQ51_RS00295	65141..66244	+	1104	WP_198866967.1	hypothetical protein	-
GKQ51_RS00300	66389..66907	+	519	WP_198866968.1	metal-dependent hydrolase	-
GKQ51_RS00305	67041..67469	+	429	WP_039800691.1	SgcJ/EcaC family oxidoreductase	-
GKQ51_RS00310	67726..68127	-	402	WP_039800694.1	helix-turn-helix domain-containing protein	Antitoxin
GKQ51_RS00315	68130..68417	-	288	WP_198866969.1	type II toxin-antitoxin system HigB family toxin	Toxin
GKQ51_RS00320	68700..69542	+	843	WP_198866970.1	alpha/beta hydrolase	-
GKQ51_RS00325	69717..70667	-	951	WP_198866971.1	homoserine kinase	-
GKQ51_RS00330	70684..70971	-	288	WP_039800707.1	DUF2782 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	63131..64492	1361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11093.78 Da
• Isoelectric Point: 9.9973

>T184278 WP_198866969.1 NZ_CP066310:c68417-68130 [Azotobacter chroococcum]
MHVITQKRIWEAKAKWPHAATALEAWYRLIKASSPQDFAEMKHLFPATDKVGPQHVFDIGGNKLRLIAVVQYRAQKVYIQ
HVLDHGEYDQGKWRE

Nucleotide

• Length: 288 bp

>T184278 NZ_CP087593:c2122667-2122416 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14909.97 Da
• Isoelectric Point: 4.4693

>AT184278 WP_039800694.1 NZ_CP066310:c68127-67726 [Azotobacter chroococcum]
MSALIKQASEHWRYVAPLLTRPANEDDYDALVEALDELLVIVGDDEDHPLATLASHMGDLIEAYDEAQRPMPKVSGVEML
RYLMQEHGLTQADLPEIGVQSVVSELLSGKRRLNVRHIRALSERFGVPADVFF

Nucleotide

• Length: 402 bp

>AT184278 NZ_CP087593:c2122426-2122244 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0C4WIL7