Detailed information of TA system TA184272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3889666..3890366
• Replicon: chromosome
• Accession: NZ_CP066306
• Organism: Pseudomonas putida strain BR-PH17

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: JET17_RS17910
• Protein ID: WP_012315363.1
• Coordinates: 3890070..3890366 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: A0A3R8WU56
• Locus tag: JET17_RS17905
• Protein ID: WP_012315362.1
• Coordinates: 3889666..3890067 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JET17_RS17885	3884798..3885619	-	822	WP_042111599.1	transporter substrate-binding domain-containing protein	-
JET17_RS17890	3885697..3886626	-	930	WP_012315359.1	FAD-binding protein	-
JET17_RS17895	3886627..3887376	-	750	WP_012315360.1	electron transfer flavoprotein subunit beta/FixA family protein	-
JET17_RS17900	3887915..3889597	+	1683	WP_080516487.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
JET17_RS17905	3889666..3890067	-	402	WP_012315362.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
JET17_RS17910	3890070..3890366	-	297	WP_012315363.1	type II toxin-antitoxin system MqsR family toxin	Toxin
JET17_RS17915	3890461..3891786	-	1326	WP_012315364.1	MFS transporter	-
JET17_RS17920	3891853..3892332	-	480	WP_012315365.1	response regulator	-
JET17_RS17925	3892503..3892979	-	477	WP_012315366.1	sigma-70 family RNA polymerase sigma factor	-
JET17_RS17930	3893184..3894362	+	1179	WP_042112070.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-127)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11200.99 Da
• Isoelectric Point: 8.4843

>T184272 WP_012315363.1 NZ_CP066306:c3890366-3890070 [Pseudomonas putida]
MEKRTPHCSLERIRALVSAGRISPTTASLRGAQALGMDYPDMLEIINGLERRDFHKSMTCHGDYRVWQDVYRPLTGKGYV
YLKLSVVDDLLIVSFKEL

Nucleotide

• Length: 297 bp

>T184272 NZ_CP087590:c1865669-1865415 [Vibrio sp. VB16]
ATGAGAATAGTATCTTTTACGGAAGCAAGAAACGGACTAAAAGCTGTTTTAGATAGTGTCGTGAATGACGCAGATACAAC
AGTTATTACCCGTCGTGACTCTGAAGATGCTGTGGTAATGTCTTTAGACTATTACAATAGCTTGATGGAAACGGTTCATT
TGCTTCGATCTCCCAAAAACGCAGAACACCTAAACCGTTCAATAGCGCAATACCGTGCAGGTAAAGTAGAAGCACGAGAA
CTAATAGATGAGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14583.67 Da
• Isoelectric Point: 6.4547

>AT184272 WP_012315362.1 NZ_CP066306:c3890067-3889666 [Pseudomonas putida]
MKCPVCGGAELAPDTRDMPYRYKGEATVIPNVSGGYCTACGEAVLSHDEAMRISTLMSAFNRQVNAAAVDPDFIVAVRKK
FDLDQREAGEIFGGGVNAFSRYENGKTRPPVALVKLFKLLDRHPELFEEVRTA

Nucleotide

• Length: 402 bp

>AT184272 NZ_CP087590:c1865422-1865156 [Vibrio sp. VB16]
ATGAGTAACCAACGCTTGTTGTCGTGGACTGATGAGGCTTGGGGTAGTTACCTTTACTGGCAGACTCAAGACAAAAAGAC
GTTGAAGCGCATAAATAAACTTATCAATGATGTGAAGCGAACGCCATTTGAAGGTATTGGTAAACCCGAGCCATTGAAAG
AAAATTTATCAGGATTCTGGTCTCGCCGTATAGATGATACTAACCGTTTAGTTTATGCAGTGGATGGGGAAACAATGACC
ATAATTTCTTGTCGTTATCACTATTAG

Structures

Antitoxin

• AlphaFold DB: A0A3R8WU56