Detailed information of TA system TA184252

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 148311..148564
• Replicon: plasmid p3
• Accession: NZ_CP066256
• Organism: Klebsiella pneumoniae strain CRKP78R

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JCR35_RS28705
• Protein ID: WP_001312851.1
• Coordinates: 148415..148564 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	148311..148370 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR35_RS28660	143344..143649	+	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-
JCR35_RS28665	143669..144034	+	366	Protein_182	type IV conjugative transfer system protein TraE	-
JCR35_RS28670	144089..144793	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JCR35_RS28675	144857..145018	+	162	Protein_184	DNA helicase	-
JCR35_RS28680	145038..145784	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JCR35_RS28685	145839..146399	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JCR35_RS28690	146531..146731	+	201	WP_015059022.1	hypothetical protein	-
JCR35_RS28695	147117..147716	+	600	WP_032083981.1	hypothetical protein	-
JCR35_RS28700	147880..148110	+	231	WP_001736714.1	hypothetical protein	-
-	148311..148370	-	60	NuclAT_1	-	Antitoxin
-	148311..148370	-	60	NuclAT_1	-	Antitoxin
-	148311..148370	-	60	NuclAT_1	-	Antitoxin
-	148311..148370	-	60	NuclAT_1	-	Antitoxin
JCR35_RS28705	148415..148564	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
JCR35_RS28710	148848..149096	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaKPC-2	-	1..149407	149407
	-	flank	IS/Tn	-	-	144089..144793	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184252 WP_001312851.1 NZ_CP066256:148415-148564 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184252 NZ_CP087578:c3923693-3923286 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT184252 NZ_CP066256:c148370-148311 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin