Detailed information of TA system TA184247

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53079..53505
• Replicon: plasmid p3
• Accession: NZ_CP066256
• Organism: Klebsiella pneumoniae strain CRKP78R

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JCR35_RS28070
• Protein ID: WP_001312861.1
• Coordinates: 53079..53237 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53281..53505 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR35_RS28035	48134..48361	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
JCR35_RS28040	48455..49141	-	687	WP_015059009.1	PAS domain-containing protein	-
JCR35_RS28045	49332..49715	-	384	WP_000124981.1	relaxosome protein TraM	-
JCR35_RS28050	49992..50639	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JCR35_RS28055	50936..51757	-	822	WP_001234445.1	DUF945 domain-containing protein	-
JCR35_RS28060	51868..52164	-	297	WP_001272251.1	hypothetical protein	-
JCR35_RS28065	52464..52841	+	378	Protein_62	hypothetical protein	-
JCR35_RS28070	53079..53237	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	53281..53505	-	225	NuclAT_0	-	Antitoxin
-	53281..53505	-	225	NuclAT_0	-	Antitoxin
-	53281..53505	-	225	NuclAT_0	-	Antitoxin
-	53281..53505	-	225	NuclAT_0	-	Antitoxin
JCR35_RS28075	53517..54236	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JCR35_RS28080	54233..54667	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JCR35_RS28085	54736..56759	-	2024	Protein_66	ParB/RepB/Spo0J family partition protein	-
JCR35_RS28090	56820..57053	-	234	WP_000006003.1	DUF905 family protein	-
JCR35_RS28095	57111..57638	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaKPC-2	-	1..149407	149407

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T184247 WP_001312861.1 NZ_CP066256:c53237-53079 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T184247 NZ_CP087578:c2979262-2979008 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT184247 NZ_CP066256:c53505-53281 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin