Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 103717..104444 | Replicon | plasmid p2 |
| Accession | NZ_CP066255 | ||
| Organism | Klebsiella pneumoniae strain CRKP78R | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | JCR35_RS27185 | Protein ID | WP_011251285.1 |
| Coordinates | 103717..104028 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | JCR35_RS27190 | Protein ID | WP_011251286.1 |
| Coordinates | 104025..104444 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JCR35_RS27145 | 98728..99238 | + | 511 | Protein_110 | hypothetical protein | - |
| JCR35_RS27150 | 99228..99668 | + | 441 | WP_011251275.1 | hypothetical protein | - |
| JCR35_RS27155 | 100093..101049 | - | 957 | WP_011251280.1 | DsbA family protein | - |
| JCR35_RS27160 | 101109..101450 | - | 342 | WP_011251281.1 | hypothetical protein | - |
| JCR35_RS27165 | 101464..101775 | - | 312 | WP_011251282.1 | hypothetical protein | - |
| JCR35_RS27170 | 101792..102226 | - | 435 | Protein_115 | thioredoxin fold domain-containing protein | - |
| JCR35_RS27175 | 102243..102940 | - | 698 | Protein_116 | IS1 family transposase | - |
| JCR35_RS27180 | 103054..103512 | + | 459 | Protein_117 | DDE-type integrase/transposase/recombinase | - |
| JCR35_RS27185 | 103717..104028 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| JCR35_RS27190 | 104025..104444 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| JCR35_RS27195 | 104591..105559 | + | 969 | WP_074428168.1 | IS5 family transposase | - |
| JCR35_RS27200 | 105661..105996 | - | 336 | Protein_121 | conjugal transfer protein TraI | - |
| JCR35_RS27205 | 106010..106798 | - | 789 | WP_040217257.1 | hypothetical protein | - |
| JCR35_RS27210 | 106819..107262 | - | 444 | WP_048333569.1 | hypothetical protein | - |
| JCR35_RS27215 | 107858..108613 | + | 756 | Protein_124 | hypothetical protein | - |
| JCR35_RS27220 | 108806..109276 | + | 471 | WP_048333570.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN | 1..215966 | 215966 | |
| - | inside | IScluster/Tn | - | - | 96808..121115 | 24307 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T184246 WP_011251285.1 NZ_CP066255:103717-104028 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T184246 NZ_CP087578:2966202-2966579 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCTCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAACAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCTCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAACAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT184246 WP_011251286.1 NZ_CP066255:104025-104444 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT184246 NZ_CP087578:2965744-2966112 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|