Detailed information of TA system TA184223

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 120980..121233
• Replicon: plasmid p3
• Accession: NZ_CP066251
• Organism: Klebsiella pneumoniae strain CRKP52R

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JCR34_RS28780
• Protein ID: WP_001312851.1
• Coordinates: 121084..121233 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	120980..121039 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR34_RS28740	116362..116706	-	345	Protein_142	IS6-like element IS26 family transposase	-
JCR34_RS28745	116758..117462	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JCR34_RS28750	117526..117687	+	162	Protein_144	DNA helicase	-
JCR34_RS28755	117707..118453	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JCR34_RS28760	118508..119068	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JCR34_RS28765	119200..119400	+	201	WP_015059022.1	hypothetical protein	-
JCR34_RS28770	119786..120385	+	600	WP_032083981.1	hypothetical protein	-
JCR34_RS28775	120549..120779	+	231	WP_001736714.1	hypothetical protein	-
-	120980..121039	-	60	NuclAT_1	-	Antitoxin
-	120980..121039	-	60	NuclAT_1	-	Antitoxin
-	120980..121039	-	60	NuclAT_1	-	Antitoxin
-	120980..121039	-	60	NuclAT_1	-	Antitoxin
JCR34_RS28780	121084..121233	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
JCR34_RS28785	121517..121765	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..122076	122076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184223 WP_001312851.1 NZ_CP066251:121084-121233 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184223 NZ_CP087570:4207964-4208620 [Escherichia coli]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGCAAAAAGCTCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGAAACGTGGCAGCACACGACGTACCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGGATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT184223 NZ_CP066251:c121039-120980 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin