Detailed information of TA system TA184219

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23197..23623
• Replicon: plasmid p3
• Accession: NZ_CP066251
• Organism: Klebsiella pneumoniae strain CRKP52R

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JCR34_RS28180
• Protein ID: WP_001312861.1
• Coordinates: 23465..23623 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23197..23421 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR34_RS28155	19064..19591	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
JCR34_RS28160	19649..19882	+	234	WP_000006003.1	DUF905 family protein	-
JCR34_RS28165	19943..21966	+	2024	Protein_27	ParB/RepB/Spo0J family partition protein	-
JCR34_RS28170	22035..22469	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JCR34_RS28175	22466..23185	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	23197..23421	+	225	NuclAT_0	-	Antitoxin
-	23197..23421	+	225	NuclAT_0	-	Antitoxin
-	23197..23421	+	225	NuclAT_0	-	Antitoxin
-	23197..23421	+	225	NuclAT_0	-	Antitoxin
JCR34_RS28180	23465..23623	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JCR34_RS28185	23861..24238	-	378	Protein_31	hypothetical protein	-
JCR34_RS28190	24538..24834	+	297	WP_001272251.1	hypothetical protein	-
JCR34_RS28195	24945..25766	+	822	WP_001234445.1	DUF945 domain-containing protein	-
JCR34_RS28200	26063..26710	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
JCR34_RS28205	26987..27370	+	384	WP_000124981.1	relaxosome protein TraM	-
JCR34_RS28210	27651..28355	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..122076	122076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T184219 WP_001312861.1 NZ_CP066251:23465-23623 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T184219 NZ_CP087570:3407451-3407554 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 225 bp

>AT184219 NZ_CP066251:23197-23421 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin