Detailed information of TA system TA184217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 100722..101449
• Replicon: plasmid p2
• Accession: NZ_CP066250
• Organism: Klebsiella pneumoniae strain CRKP52R

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: JCR34_RS27530
• Protein ID: WP_011251285.1
• Coordinates: 100722..101033 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JCR34_RS27535
• Protein ID: WP_011251286.1
• Coordinates: 101030..101449 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR34_RS27510	96241..96735	-	495	WP_004212794.1	thermonuclease family protein	-
JCR34_RS27515	96913..97862	+	950	Protein_111	DUF1173 family protein	-
JCR34_RS27520	99248..99945	-	698	Protein_112	IS1 family transposase	-
JCR34_RS27525	100059..100517	+	459	Protein_113	DDE-type integrase/transposase/recombinase	-
JCR34_RS27530	100722..101033	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
JCR34_RS27535	101030..101449	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
JCR34_RS27540	101596..102564	+	969	WP_074428168.1	IS5 family transposase	-
JCR34_RS27545	102666..103001	-	336	Protein_117	conjugal transfer protein TraI	-
JCR34_RS27550	103015..103803	-	789	WP_040217257.1	hypothetical protein	-
JCR34_RS27555	103824..104267	-	444	WP_048333569.1	hypothetical protein	-
JCR34_RS27560	104863..105618	+	756	Protein_120	hypothetical protein	-
JCR34_RS27565	105811..106281	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA	1..193221	193221
	-	inside	IScluster/Tn	-	-	94055..119078	25023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T184217 WP_011251285.1 NZ_CP066250:100722-101033 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T184217 NZ_CP087570:3233003-3233254 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT184217 WP_011251286.1 NZ_CP066250:101030-101449 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT184217 NZ_CP087570:3233251-3233505 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1