Detailed information of TA system TA184188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3809..4467
• Replicon: plasmid pG20AB010-1
• Accession: NZ_CP066233
• Organism: Acinetobacter baumannii strain G20AB010

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JCR18_RS19280
• Protein ID: WP_000312250.1
• Coordinates: 4108..4467 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JCR18_RS19275
• Protein ID: WP_001096429.1
• Coordinates: 3809..4108 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JCR18_RS19250	517..1026	+	510	WP_001043199.1	hypothetical protein	-
JCR18_RS19255	1107..1661	+	555	WP_000790085.1	hypothetical protein	-
JCR18_RS19260	1711..2247	+	537	WP_000731981.1	hypothetical protein	-
JCR18_RS19265	2870..3352	+	483	WP_001052677.1	hypothetical protein	-
JCR18_RS19270	3445..3708	-	264	WP_000110863.1	hypothetical protein	-
JCR18_RS19275	3809..4108	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
JCR18_RS19280	4108..4467	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JCR18_RS19285	4668..5234	+	567	WP_041171756.1	hypothetical protein	-
JCR18_RS19290	5283..5465	+	183	WP_000373385.1	hypothetical protein	-
JCR18_RS19295	5532..6230	+	699	WP_000873190.1	hypothetical protein	-
JCR18_RS19300	6369..6752	+	384	WP_000654349.1	hypothetical protein	-
JCR18_RS19305	6819..7577	+	759	WP_001053129.1	hypothetical protein	-
JCR18_RS19310	8647..8961	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73360	73360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T184188 WP_000312250.1 NZ_CP066233:c4467-4108 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T184188 NZ_CP087564:2027917-2028291 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCTCATCTCCCGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCACCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT184188 WP_001096429.1 NZ_CP066233:c4108-3809 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT184188 NZ_CP087564:2027459-2027827 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAAAGGGCTGACCTGCAAAGCCGACACCCTCGGTAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA