Detailed information of TA system TA184166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 491990..492609
• Replicon: chromosome
• Accession: NZ_CP066203
• Organism: Methylacidimicrobium sp. B4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MacB4_RS02395
• Protein ID: WP_206864282.1
• Coordinates: 492313..492609 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MacB4_RS02390
• Protein ID: WP_024807448.1
• Coordinates: 491990..492298 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MacB4_RS02360	487689..489062	+	1374	WP_206864280.1	3-isopropylmalate dehydratase	-
MacB4_RS02365	489059..489418	+	360	WP_206864281.1	hypothetical protein	-
MacB4_RS02375	489635..489796	-	162	Protein_461	recombinase family protein	-
MacB4_RS02380	489955..491214	+	1260	WP_206864906.1	transposase	-
MacB4_RS02385	491233..491928	-	696	Protein_463	recombinase family protein	-
MacB4_RS02390	491990..492298	-	309	WP_024807448.1	HigA family addiction module antidote protein	Antitoxin
MacB4_RS02395	492313..492609	-	297	WP_206864282.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MacB4_RS02400	492606..492878	-	273	WP_206864907.1	transposase	-
MacB4_RS02405	492903..493319	-	417	WP_206864283.1	hypothetical protein	-
MacB4_RS02410	493316..493540	-	225	WP_206864284.1	DUF3018 family protein	-
MacB4_RS02415	493711..494220	+	510	WP_206864285.1	RDD family protein	-
MacB4_RS02420	494273..494695	-	423	WP_206864286.1	CopD family protein	-
MacB4_RS02425	494692..495024	-	333	WP_206864287.1	DUF3817 domain-containing protein	-
MacB4_RS02430	495163..496350	-	1188	WP_206864288.1	cation:proton antiporter	-
MacB4_RS02435	496554..497366	+	813	WP_206864289.1	L,D-transpeptidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	489943..491214	1271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11436.81 Da
• Isoelectric Point: 6.4844

>T184166 WP_206864282.1 NZ_CP066203:c492609-492313 [Methylacidimicrobium sp. B4]
MIVGFRDEWLRAFFVDDAQSRNIPSDLEARLFRKLQMIDDATTDHDLRVPPSNHFEKLKGNLAGLHSIRVSKQYRLVFRS
DGERGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T184166 NZ_CP087392:3228557-3228961 [Pectobacterium parvum]
ATGCTGAAATATCTGCTCGATACCAATATCTGTATTTACACCATCAAGAACAAACCGCAGGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGTTGATGGAACTGATCTATGGTGCGGAGAAATCGGTAAACCCAG
AAAAGAATCTCGCCGTGATTGAAGGGTTTTCTGCGCGTCTGGAAGTTCAAAACTACGGTTTTGACGCTGCCGTACATACC
GGACAAATTCGTGCGGAACTGGCAAAACAAGGAACACCCATCGGCCCGTATGATGCCATGCTCGCGGGTCATGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAATTTGAGCGTGTGCCTGGGTTACGAGTGGAAAACTGGATAAGCC
GATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11520.36 Da
• Isoelectric Point: 10.9969

>AT184166 WP_024807448.1 NZ_CP066203:c492298-491990 [Methylacidimicrobium sp. B4]
MLTTKRKPASVGEILTEEFMEPMGLTQGALAEAMGVQRKHVNELCSNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
EVMNTPKERARVERARPVKKAA

Nucleotide

• Length: 309 bp

>AT184166 NZ_CP087392:3228327-3228557 [Pectobacterium parvum]
ATGGAGAAAACTACAGTATTTAAAAGCAATCGCAGCCAGGCGGTACGGCTCCCCAAAGCCGTTGCGCTGCCCGATGATGT
CAAGCAGGTTGATATCGTTGCGATTGGTCGCACACGTATCATTAGTCCGGCAGGGGAAAGCTGGGACAGTTGGTTCGAAG
AACAAGGAGTAACACCTGACTTTATAATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTCTGA