Detailed information of TA system TA184116

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132529..132782
• Replicon: plasmid unnamed2
• Accession: NZ_CP066153
• Organism: Klebsiella pneumoniae strain KP697

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JDZ44_RS29100
• Protein ID: WP_001312851.1
• Coordinates: 132633..132782 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	132529..132588 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDZ44_RS29060 (127911)	127911..128255	-	345	Protein_162	IS6-like element IS26 family transposase	-
JDZ44_RS29065 (128307)	128307..129011	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JDZ44_RS29070 (129036)	129036..129236	+	201	WP_072354025.1	hypothetical protein	-
JDZ44_RS29075 (129256)	129256..130002	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
JDZ44_RS29080 (130057)	130057..130617	+	561	WP_000139328.1	fertility inhibition protein FinO	-
JDZ44_RS29085 (130749)	130749..130949	+	201	WP_015059022.1	hypothetical protein	-
JDZ44_RS29090 (131335)	131335..131934	+	600	WP_032083981.1	PIN domain-containing protein	-
JDZ44_RS29095 (131996)	131996..132328	+	333	WP_152916585.1	hypothetical protein	-
- (132529)	132529..132588	-	60	NuclAT_1	-	Antitoxin
- (132529)	132529..132588	-	60	NuclAT_1	-	Antitoxin
- (132529)	132529..132588	-	60	NuclAT_1	-	Antitoxin
- (132529)	132529..132588	-	60	NuclAT_1	-	Antitoxin
JDZ44_RS29100 (132633)	132633..132782	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
JDZ44_RS29105 (133066)	133066..133314	+	249	WP_000083837.1	replication regulatory protein RepA	-
JDZ44_RS29110 (133429)	133429..133613	+	185	Protein_172	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133625	133625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184116 WP_001312851.1 NZ_CP066153:132633-132782 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184116 NZ_CP087377:c883282-882905 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT184116 NZ_CP066153:c132588-132529 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin