Detailed information of TA system TA184083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4915446..4916048
• Replicon: chromosome
• Accession: NZ_CP066144
• Organism: Escherichia coli strain ZLWT

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: ICI43_RS23465
• Protein ID: WP_000897302.1
• Coordinates: 4915737..4916048 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ICI43_RS23460
• Protein ID: WP_000356397.1
• Coordinates: 4915446..4915736 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI43_RS23435	4911519..4912421	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
ICI43_RS23440	4912418..4913053	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ICI43_RS23445	4913050..4913979	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
ICI43_RS23450	4914195..4914413	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
ICI43_RS23455	4914809..4915087	-	279	WP_001296612.1	hypothetical protein	-
ICI43_RS23460	4915446..4915736	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ICI43_RS23465	4915737..4916048	-	312	WP_000897302.1	hypothetical protein	Toxin
ICI43_RS23470	4916278..4917186	+	909	WP_001305059.1	alpha/beta hydrolase	-
ICI43_RS23475	4917250..4918191	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ICI43_RS23480	4918236..4918673	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ICI43_RS23485	4918670..4919542	-	873	WP_000920762.1	virulence factor BrkB family protein	-
ICI43_RS23490	4919536..4920135	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T184083 WP_000897302.1 NZ_CP066144:c4916048-4915737 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T184083 NZ_CP087329:37025-37321 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTGCCTGAATTTGATGAATGGCTAGATGGTCTTAAAGACAATATGACTCGAATCCGCTTGAA
TCGTCGATTAGATAAGGTCCAACGTGGTAATTGGGGTGACATTAAACCACTCCAAGATGGTGTCTGGGAAATGAGAGAGT
TCTTTGGTGCTGGTTGGCGGATGTACTACATCCAACATGGTGACGTTGTGATCGTGATGCTTGGTGGTGGTGAAAAATCT
ACACAGAAAGATGACATCAAGAAAGCGGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT184083 WP_000356397.1 NZ_CP066144:c4915736-4915446 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT184083 NZ_CP087329:37323-37613 [Acinetobacter baumannii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGATATTGTAATGTACCTCAA
TATGGTTCTTGAAGAAAATGATCCTGCTGAATTAGCGCACGCACTTGGTGTAATTGCTAAAGCAAGAGGTATGACCCAAA
TTGCGAAAGAAGCAGGTATTGGACGAGAAGCCCTTTACAAAGCACTTCGTCAAGACTCAACGCCTCGTTTTGATACGATC
AACCGTGTTGTGAATGCTTTAGGTTTAAAATTGACAGTTCAACATACTTAA

Structures

Toxin

• AlphaFold DB: S1P416