Detailed information of TA system TA184049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4132323..4132963
• Replicon: chromosome
• Accession: NZ_CP066143
• Organism: Klebsiella pneumoniae U-0608239

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: I6N99_RS20590
• Protein ID: WP_016529833.1
• Coordinates: 4132616..4132963 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6N99_RS20585
• Protein ID: WP_040181955.1
• Coordinates: 4132323..4132616 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6N99_RS20565 (4128522)	4128522..4129280	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
I6N99_RS20570 (4129330)	4129330..4130160	-	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
I6N99_RS20575 (4130211)	4130211..4130540	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
I6N99_RS20580 (4130745)	4130745..4132253	+	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
I6N99_RS20585 (4132323)	4132323..4132616	-	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
I6N99_RS20590 (4132616)	4132616..4132963	-	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6N99_RS20595 (4133139)	4133139..4135586	-	2448	WP_002920561.1	glycogen phosphorylase	-
I6N99_RS20600 (4135604)	4135604..4137037	-	1434	WP_002920564.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T184049 WP_016529833.1 NZ_CP066143:c4132963-4132616 [Klebsiella pneumoniae U-0608239]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T184049 NZ_CP087300:1268927-1269109 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT184049 WP_040181955.1 NZ_CP066143:c4132616-4132323 [Klebsiella pneumoniae U-0608239]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT184049 NZ_CP087300:1269202-1269606 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: W9BBY1