Detailed information of TA system TA183994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 6617592..6618196
• Replicon: chromosome
• Accession: NZ_CP066123
• Organism: Pseudomonas sp. MPFS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I9018_RS30465
• Protein ID: WP_241108235.1
• Coordinates: 6617592..6617906 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9018_RS30470
• Protein ID: WP_241108236.1
• Coordinates: 6617906..6618196 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9018_RS30430 (I9018_30330)	6613328..6613651	+	324	WP_022641629.1	DUF3392 domain-containing protein	-
I9018_RS30435 (I9018_30335)	6613681..6614229	+	549	WP_241108232.1	RNA 2'-phosphotransferase	-
I9018_RS30440 (I9018_30340)	6614284..6615009	-	726	WP_241108233.1	tRNA (guanosine(46)-N7)-methyltransferase TrmB	-
I9018_RS30445 (I9018_30345)	6615019..6615813	-	795	WP_068575475.1	thiazole synthase	-
I9018_RS30450 (I9018_30350)	6615999..6616199	-	201	WP_164487305.1	sulfur carrier protein ThiS	-
I9018_RS30455 (I9018_30355)	6616271..6616651	-	381	WP_068575474.1	DUF423 domain-containing protein	-
I9018_RS30460 (I9018_30360)	6616726..6617448	+	723	WP_241108234.1	monofunctional biosynthetic peptidoglycan transglycosylase	-
I9018_RS30465 (I9018_30365)	6617592..6617906	+	315	WP_241108235.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9018_RS30470 (I9018_30370)	6617906..6618196	+	291	WP_241108236.1	helix-turn-helix domain-containing protein	Antitoxin
I9018_RS30475 (I9018_30375)	6618295..6619149	-	855	WP_068575472.1	RNA polymerase sigma factor RpoH	-
I9018_RS30480 (I9018_30380)	6619263..6620285	-	1023	WP_068575471.1	permease-like cell division protein FtsX	-
I9018_RS30485 (I9018_30385)	6620282..6620953	-	672	WP_068575470.1	cell division ATP-binding protein FtsE	-
I9018_RS30490 (I9018_30390)	6620950..6622494	-	1545	WP_241108237.1	signal recognition particle-docking protein FtsY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11780.47 Da
• Isoelectric Point: 9.6229

>T183994 WP_241108235.1 NZ_CP066123:6617592-6617906 [Pseudomonas sp. MPFS]
MIFIESPIFTEDLKSLLSDESYRAFQEYLAENPTAGDIIQGTNGLRKVRWGANGRGKSGGARVIYYHVTAAYQIRLILIY
RKGIKDDLSPSEKKILSEINKGWQ

Nucleotide

• Length: 315 bp

>T183994 NZ_CP087290:1453795-1454172 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10969.54 Da
• Isoelectric Point: 8.8614

>AT183994 WP_241108236.1 NZ_CP066123:6617906-6618196 [Pseudomonas sp. MPFS]
MEKNLFNRLVESMTQMDEIQRGERSPSREFHIDALKVKMIRKASGLSQARFAKAIDVPVGTLQNWEQGRREPEGPARALL
RAIHNDPEHVLAALEH

Nucleotide

• Length: 291 bp

>AT183994 NZ_CP087290:1453337-1453705 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCTGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA