Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 564559..565253 Replicon chromosome
Accession NZ_CP066123
Organism Pseudomonas sp. MPFS

Toxin (Protein)


Gene name higB Uniprot ID U6ZZC4
Locus tag I9018_RS02475 Protein ID WP_022640299.1
Coordinates 564559..564855 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6G6ZSS2
Locus tag I9018_RS02480 Protein ID WP_068578624.1
Coordinates 564852..565253 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9018_RS02440 (I9018_02450) 560191..560736 - 546 WP_124345577.1 hypothetical protein -
I9018_RS02445 (I9018_02455) 560733..560987 - 255 WP_068578636.1 acyl carrier protein -
I9018_RS02450 (I9018_02460) 560997..561269 - 273 WP_068578634.1 phosphopantetheine-binding protein -
I9018_RS02455 (I9018_02465) 561238..562059 - 822 WP_241108654.1 lysophospholipid acyltransferase family protein -
I9018_RS02460 (I9018_02470) 562035..562763 - 729 WP_241108655.1 beta-ketoacyl synthase chain length factor -
I9018_RS02465 (I9018_02475) 563074..563844 + 771 WP_068578628.1 ParA family protein -
I9018_RS02470 (I9018_02480) 563896..564330 - 435 WP_241108656.1 thioredoxin TrxC -
I9018_RS02475 (I9018_02485) 564559..564855 + 297 WP_022640299.1 type II toxin-antitoxin system HigB family toxin Toxin
I9018_RS02480 (I9018_02490) 564852..565253 + 402 WP_068578624.1 transcriptional regulator Antitoxin
I9018_RS02485 (I9018_02495) 565255..566718 - 1464 WP_241108657.1 YdiU family protein -
I9018_RS02490 (I9018_02500) 566788..570114 - 3327 WP_241108658.1 mechanosensitive channel MscK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-90)

Antitoxin

Antitoxin HigA

(15-133)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11566.41 Da        Isoelectric Point: 10.3534

>T183986 WP_022640299.1 NZ_CP066123:564559-564855 [Pseudomonas sp. MPFS]
MHIITAKRIWQAKEQWPQTARALDTWYRKAKGARPEDFSDMKALFPAVDKVGRFHVFDIGGNKLRLIALVRYGVQRLYIR
HVLDHASYDKGLWKEEIE

Download         Length: 297 bp

>T183986 NZ_CP087288:168734-169150 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14852.94 Da        Isoelectric Point: 4.4569

>AT183986 WP_068578624.1 NZ_CP066123:564852-565253 [Pseudomonas sp. MPFS]
MSVLVSQAAEHWQFVAPLLRKPRTEADYDALALALDELLDIMGEDEAHPLASLVDIIGDWIEAYDEEHRPIPPVSGVEVL
RSLMHEHGLTQSDLPGVGAQSVVSEVLSGKRQLNLRQVRWLSERFKVAMEVFA

Download         Length: 402 bp

>AT183986 NZ_CP087288:168507-168737 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G6ZSS4

Antitoxin

Source ID Structure
AlphaFold DB A0A6G6ZSS2

References