Detailed information of TA system TA183981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 69492..70077
• Replicon: plasmid unnamed map unlocalized
• Accession: NZ_CP066122
• Organism: Acinetobacter bereziniae strain GD0320

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: I9190_RS21870
• Protein ID: WP_000897307.1
• Coordinates: 69757..70077 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9190_RS21865
• Protein ID: WP_000369781.1
• Coordinates: 69492..69764 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9190_RS21850 (I9190_21850)	65097..65981	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
I9190_RS21855 (I9190_21855)	66037..67512	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
I9190_RS21860 (I9190_21860)	68076..69401	+	1326	WP_079393426.1	ISNCY family transposase	-
I9190_RS21865 (I9190_21865)	69492..69764	-	273	WP_000369781.1	NadS family protein	Antitoxin
I9190_RS21870 (I9190_21870)	69757..70077	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9190_RS21875 (I9190_21875)	70217..70636	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
I9190_RS21880 (I9190_21880)	70716..70997	-	282	WP_000985609.1	putative addiction module antidote protein	-
I9190_RS21885 (I9190_21885)	70998..71300	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I9190_RS21890 (I9190_21890)	71449..71979	+	531	WP_000172759.1	hypothetical protein	-
I9190_RS21895 (I9190_21895)	72068..72382	-	315	WP_004728120.1	BrnA antitoxin family protein	-
I9190_RS21900 (I9190_21900)	72369..72656	-	288	WP_000438825.1	BrnT family toxin	-
I9190_RS21905 (I9190_21905)	72886..73491	-	606	WP_000447788.1	LysE family translocator	-
I9190_RS21910 (I9190_21910)	73541..74368	-	828	WP_000073658.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / cmlB1 / mph(E) / msr(E) / blaOXA-58	-	1..122921	122921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T183981 WP_000897307.1 NZ_CP066122:c70077-69757 [Acinetobacter bereziniae]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T183981 NZ_CP087287:5051425-5051736 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT183981 WP_000369781.1 NZ_CP066122:c69764-69492 [Acinetobacter bereziniae]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT183981 NZ_CP087287:5051737-5052027 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3