Detailed information of TA system TA183976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 9054..9639
• Replicon: plasmid unnamed map unlocalized
• Accession: NZ_CP066120
• Organism: Acinetobacter bereziniae strain GD03185

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: I9192_RS22030
• Protein ID: WP_000897307.1
• Coordinates: 9319..9639 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9192_RS22025
• Protein ID: WP_000369781.1
• Coordinates: 9054..9326 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9192_RS22010 (I9192_22005)	4659..5543	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
I9192_RS22015 (I9192_22010)	5599..7074	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
I9192_RS22020 (I9192_22015)	7638..8963	+	1326	WP_200150442.1	ISNCY family transposase	-
I9192_RS22025 (I9192_22020)	9054..9326	-	273	WP_000369781.1	NadS family protein	Antitoxin
I9192_RS22030 (I9192_22025)	9319..9639	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9192_RS22035 (I9192_22030)	9763..11088	-	1326	WP_200150442.1	ISNCY family transposase	-
I9192_RS22040 (I9192_22035)	11237..11518	-	282	WP_000985609.1	putative addiction module antidote protein	-
I9192_RS22045 (I9192_22040)	11519..11821	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I9192_RS22050 (I9192_22045)	11976..12617	-	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
I9192_RS22055 (I9192_22050)	12683..13870	+	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / tet(39) / blaCARB-2 / sul1	-	1..43270	43270
	-	inside	IScluster/Tn	mph(E) / msr(E) / tet(39) / blaCARB-2 / sul1	-	3082..21002	17920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T183976 WP_000897307.1 NZ_CP066120:c9639-9319 [Acinetobacter bereziniae]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T183976 NZ_CP087287:c3888196-3887789 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT183976 WP_000369781.1 NZ_CP066120:c9326-9054 [Acinetobacter bereziniae]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT183976 NZ_CP087287:c3888443-3888177 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3