Detailed information of TA system TA183960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1224983..1225644
• Replicon: chromosome
• Accession: NZ_CP066108
• Organism: Enterobacter hormaechei strain Eho-7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GVI68_RS05895
• Protein ID: WP_226865158.1
• Coordinates: 1225399..1225644 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: F5RUL2
• Locus tag: GVI68_RS05890
• Protein ID: WP_006809705.1
• Coordinates: 1224983..1225402 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI68_RS05855 (GVI68_05845)	1220759..1221649	+	891	WP_006809710.1	oxidoreductase	-
GVI68_RS05860 (GVI68_05850)	1221703..1222137	-	435	WP_006809709.1	hypothetical protein	-
GVI68_RS05865 (GVI68_05855)	1222134..1222490	-	357	WP_006809708.1	DUF2778 domain-containing protein	-
GVI68_RS05870 (GVI68_05860)	1222515..1223675	-	1161	WP_039273381.1	IS30-like element ISCfr4 family transposase	-
GVI68_RS05875 (GVI68_05865)	1223724..1224215	-	492	WP_039273384.1	Hcp family type VI secretion system effector	-
GVI68_RS05880 (GVI68_05870)	1224559..1224782	-	224	Protein_1140	glutamate-cysteine ligase family protein	-
GVI68_RS05885 (GVI68_05875)	1224778..1224930	+	153	Protein_1141	ketopantoate reductase C-terminal domain-containing protein	-
GVI68_RS05890 (GVI68_05880)	1224983..1225402	-	420	WP_006809705.1	helix-turn-helix domain-containing protein	Antitoxin
GVI68_RS05895 (GVI68_05885)	1225399..1225644	-	246	WP_226865158.1	type II toxin-antitoxin system HigB family toxin	Toxin
GVI68_RS05900 (GVI68_05890)	1225866..1226615	+	750	WP_032673600.1	hypothetical protein	-
GVI68_RS05905 (GVI68_05895)	1226827..1227282	-	456	WP_006809702.1	hypothetical protein	-
GVI68_RS05910 (GVI68_05900)	1227569..1228450	-	882	WP_039273386.1	LysR family transcriptional regulator	-
GVI68_RS05915 (GVI68_05905)	1228556..1229335	+	780	WP_003858848.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1222515..1223675	1160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (2-71)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 10088.63 Da
• Isoelectric Point: 9.8340

>T183960 WP_226865158.1 NZ_CP066108:c1225644-1225399 [Enterobacter hormaechei]
MDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIEVGGGHLRVMFFADFERGKIFIKHISTHAEYDKLTDYYRRTK
E

Nucleotide

• Length: 246 bp

>T183960 NZ_CP087282:c126198-125782 [Leclercia adecarboxylata]
GTGAACAAAACGTATATGCTGGACACCAACATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTACTGCGCAACCAGCGCATCGTGGTCTCGGCCATTACTTACTCCGAGATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCCCCGCGCCACACCGAGCTGGTTGACGAGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGTGCC
GCGGTGGATGCATCCACGGACGTTAAAGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGCTCATTGC
CGGGCACGCCATTGCGGCCGGCGCCATCCTGGTGACGAATAATGTGAGGGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTTAACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15447.51 Da
• Isoelectric Point: 4.5951

>AT183960 WP_006809705.1 NZ_CP066108:c1225402-1224983 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSTPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT183960 NZ_CP087282:c126425-126195 [Leclercia adecarboxylata]
ATGCGCACAGTTTCTATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGACCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
AAAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCACCAACGAAGGACGGGTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7T1QZI6