Detailed information of TA system TA183941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1224988..1225649
• Replicon: chromosome
• Accession: NZ_CP066101
• Organism: Enterobacter hormaechei strain Eho-8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GVI69_RS05895
• Protein ID: WP_226865158.1
• Coordinates: 1225404..1225649 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: F5RUL2
• Locus tag: GVI69_RS05890
• Protein ID: WP_006809705.1
• Coordinates: 1224988..1225407 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI69_RS05855 (GVI69_05845)	1220767..1221657	+	891	WP_006809710.1	oxidoreductase	-
GVI69_RS05860 (GVI69_05850)	1221711..1222145	-	435	WP_006809709.1	hypothetical protein	-
GVI69_RS05865 (GVI69_05855)	1222142..1222498	-	357	WP_006809708.1	DUF2778 domain-containing protein	-
GVI69_RS05870 (GVI69_05860)	1222523..1223679	-	1157	Protein_1138	IS30 family transposase	-
GVI69_RS05875 (GVI69_05865)	1223728..1224219	-	492	WP_039273384.1	Hcp family type VI secretion system effector	-
GVI69_RS05880 (GVI69_05870)	1224564..1224787	-	224	Protein_1140	glutamate-cysteine ligase family protein	-
GVI69_RS05885 (GVI69_05875)	1224783..1224935	+	153	Protein_1141	ketopantoate reductase C-terminal domain-containing protein	-
GVI69_RS05890 (GVI69_05880)	1224988..1225407	-	420	WP_006809705.1	helix-turn-helix domain-containing protein	Antitoxin
GVI69_RS05895 (GVI69_05885)	1225404..1225649	-	246	WP_226865158.1	type II toxin-antitoxin system HigB family toxin	Toxin
GVI69_RS05900 (GVI69_05890)	1226835..1227290	-	456	WP_006809702.1	hypothetical protein	-
GVI69_RS05905 (GVI69_05895)	1227577..1228458	-	882	WP_039273386.1	LysR family transcriptional regulator	-
GVI69_RS05910 (GVI69_05900)	1228564..1229343	+	780	WP_003858848.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (2-71)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 10088.63 Da
• Isoelectric Point: 9.8340

>T183941 WP_226865158.1 NZ_CP066101:c1225649-1225404 [Enterobacter hormaechei]
MDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIEVGGGHLRVMFFADFERGKIFIKHISTHAEYDKLTDYYRRTK
E

Nucleotide

• Length: 246 bp

>T183941 NZ_CP087272:c5032225-5031308 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15447.51 Da
• Isoelectric Point: 4.5951

>AT183941 WP_006809705.1 NZ_CP066101:c1225407-1224988 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSTPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT183941 NZ_CP087272:c5032989-5032231 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7T1QZI6