Detailed information of TA system TA183933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3862006..3862681
• Replicon: chromosome
• Accession: NZ_CP066094
• Organism: Enterobacter hormaechei strain Eho-E1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I9464_RS18760
• Protein ID: WP_032619177.1
• Coordinates: 3862006..3862305 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: I9464_RS18765
• Protein ID: WP_015571639.1
• Coordinates: 3862316..3862681 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9464_RS18740 (I9464_18725)	3857138..3857755	-	618	WP_032634496.1	DUF1819 family protein	-
I9464_RS18745 (I9464_18730)	3857919..3858887	-	969	WP_000654804.1	IS5-like element IS903B family transposase	-
I9464_RS18750 (I9464_18735)	3858950..3860575	+	1626	WP_230199853.1	ATP-binding cassette domain-containing protein	-
I9464_RS18755 (I9464_18740)	3860556..3861755	+	1200	WP_017382757.1	HlyD family efflux transporter periplasmic adaptor subunit	-
I9464_RS18760 (I9464_18745)	3862006..3862305	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9464_RS18765 (I9464_18750)	3862316..3862681	+	366	WP_015571639.1	HigA family addiction module antitoxin	Antitoxin
I9464_RS18770 (I9464_18755)	3862708..3863889	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
I9464_RS18775 (I9464_18760)	3863910..3864797	-	888	WP_032619173.1	LysR family transcriptional regulator	-
I9464_RS18780 (I9464_18765)	3864895..3865497	+	603	WP_017382759.1	short chain dehydrogenase	-
I9464_RS18785 (I9464_18770)	3865494..3866225	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3857919..3858842	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T183933 WP_032619177.1 NZ_CP066094:3862006-3862305 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T183933 NZ_CP087272:350588-350935 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT183933 WP_015571639.1 NZ_CP066094:3862316-3862681 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT183933 NZ_CP087272:350935-351234 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9