Detailed information of TA system TA183920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 36832..37562
• Replicon: chromosome
• Accession: NZ_CP066094
• Organism: Enterobacter hormaechei strain Eho-E1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: I9464_RS00170
• Protein ID: WP_023302825.1
• Coordinates: 36832..37146 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9464_RS00175
• Protein ID: WP_032620773.1
• Coordinates: 37146..37562 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9464_RS00150 (I9464_00150)	32498..33583	+	1086	Protein_29	cellulase family glycosylhydrolase	-
I9464_RS00155 (I9464_00155)	33489..34673	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
I9464_RS00160 (I9464_00160)	34849..35682	-	834	WP_032620535.1	DMT family transporter	-
I9464_RS00165 (I9464_00165)	35745..36191	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
I9464_RS00170 (I9464_00175)	36832..37146	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
I9464_RS00175 (I9464_00180)	37146..37562	+	417	WP_032620773.1	helix-turn-helix domain-containing protein	Antitoxin
I9464_RS00180 (I9464_00185)	37709..37807	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
I9464_RS00185 (I9464_00190)	37914..39602	+	1689	WP_023302827.1	acetolactate synthase large subunit	-
I9464_RS00190 (I9464_00195)	39606..39893	+	288	WP_015570113.1	acetolactate synthase small subunit	-
I9464_RS00195 (I9464_00200)	40019..40612	+	594	WP_003861054.1	transcriptional regulator UhpA	-
I9464_RS00200 (I9464_00205)	40609..42114	+	1506	WP_032634219.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T183920 WP_023302825.1 NZ_CP066094:36832-37146 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T183920 NZ_CP087226:3423473-3423877 [Dickeya fangzhongdai]
ATGCTGAAATATCTGCTTGATACCAATATCTGTATTTACACCATCAAGAACAAACCACAGGAAGTCAGGGACGCTTTCTA
CCGTTATTACGGGCAGTTTGCTATCAGTGCTATCACGCTGATGGAGCTGATTTATGGCGCGGAGAAATCGGTAAACCCAG
AAAAGAATCTTGCCGTGATTGAAGGGTTTTCTGCGCGTCTGGAAGTGCAAACCTATGGTTTTGACGCTGCCGTGCATACC
GGCCAAATTCGTGCGGAACTGGCAAGACAAGGAACCCCCATCGGCCCATATGATGCCATGCTAGCGGGTCACGCTCGATC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAATTTGAGCGTGTGCCTGGGCTACGAGTGGAAAACTGGGTCAGTC
GATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15249.77 Da
• Isoelectric Point: 5.2068

>AT183920 WP_032620773.1 NZ_CP066094:37146-37562 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 417 bp

>AT183920 NZ_CP087226:3423243-3423473 [Dickeya fangzhongdai]
ATGGAAAAAACCACAGTATTTAAAAGCAATCGCAGTCAAGCAGTACGACTCCCCAAAGCCGTTGCACTGCCCGATGATGT
TAAGCAGGTTGATATCGTCGCGATTGGTCGCACACGTATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGAAGGGGTAACGCCTGACTTCATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTCTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2