Detailed information of TA system TA183912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2648158..2648885
• Replicon: chromosome
• Accession: NZ_CP066089
• Organism: Citrobacter koseri strain FDAARGOS_1029

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A8AL02
• Locus tag: I6H86_RS12335
• Protein ID: WP_012133872.1
• Coordinates: 2648577..2648885 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6H86_RS12330
• Protein ID: WP_012133871.1
• Coordinates: 2648158..2648577 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H86_RS12305	2643354..2644283	+	930	WP_012133862.1	1,4-dihydroxy-2-naphthoate polyprenyltransferase	-
I6H86_RS12310	2644376..2644861	+	486	WP_012133864.1	ribonuclease E activity regulator RraA	-
I6H86_RS12315	2645027..2645272	-	246	WP_012133867.1	septal ring assembly protein ZapB	-
I6H86_RS12320	2645663..2646508	+	846	WP_012133869.1	aquaporin	-
I6H86_RS12325	2646532..2648040	+	1509	WP_012133870.1	glycerol kinase GlpK	-
I6H86_RS12330	2648158..2648577	-	420	WP_012133871.1	helix-turn-helix domain-containing protein	Antitoxin
I6H86_RS12335	2648577..2648885	-	309	WP_012133872.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6H86_RS12340	2649026..2650036	+	1011	WP_012133873.1	class II fructose-bisphosphatase	-
I6H86_RS12345	2650132..2650878	+	747	WP_012133874.1	ferredoxin--NADP(+) reductase	-
I6H86_RS12350	2650954..2651400	-	447	WP_012133875.1	DUF805 domain-containing protein	-
I6H86_RS12355	2651501..2652097	+	597	WP_012133876.1	YiiQ family protein	-
I6H86_RS12360	2652212..2652979	+	768	WP_024130662.1	triose-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 12368.15 Da
• Isoelectric Point: 10.0486

>T183912 WP_012133872.1 NZ_CP066089:c2648885-2648577 [Citrobacter koseri]
MHVISKEPFEEAAKRYPNDSLAIRSLYRLMRETDFSSPTEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHISTHAEYDKLTRYYRENK

Nucleotide

• Length: 309 bp

>T183912 NZ_CP087226:425847-426422 [Dickeya fangzhongdai]
ATGACCCATACACCTTATCCTGTCGTGCTAGATGCGTGCGTAATTTATCCATCTCTCCTGCGGGATTTATTAATCCACTT
AGGACTAACAGGGCTGTATCAGCCTAAGTGGACGGCCATCATCGAGGATGAATGGCAACGCAATCTACTGCTGAATAGAC
CTGATTTAACCCCGGAGCAAATCTCGCGGACACGCGAGCTGATGAATCAGGCTATTCCTGATGCGATGATTACTGGGTTT
GAAGCGCTCATTCCAGGCATCACGCTCCCTGATCCTGATGATCAGCATGTGGTTGCAGCCGCCATTCGCAGTAACGCTGA
AATCATCGTTACGTTTAACCTGAAAGATTTTCCCCATCCCGAGATCGATAACTTTGGCATTGAAGCGCTACACCCGGATG
ATTTCATCACAGACCTGCTCGATTTGAATCAGGCGCTTGTGTTATCCGCAGTAAGCAGGCAGCGTCACAGCATGAGAAAA
CCGCCTAAATCGGTTGATGAGTATCTTGATGCGTTACTCCGGCAGAGCTTACCGCAAACAGTAAAAGAATTGAGTAAATT
CCACGCCCTGATTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15380.38 Da
• Isoelectric Point: 4.3485

>AT183912 WP_012133871.1 NZ_CP066089:c2648577-2648158 [Citrobacter koseri]
MNTDTAKAIEATKQLVAAVPFLGGSSSESDYREALSLVDYLIENDDENPLIDFLASKIADYEDNSDRFAEFNKGIAEMPV
GVALLRTLIDQYKLSYSDLKEEIGSKSLVSQILSGQRSLTIAHIKALSTRFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT183912 NZ_CP087226:425386-425847 [Dickeya fangzhongdai]
ATGACAAACTCAATTCTTGATAGCCTGAGCCTTCCGGCACCAGGGGAGATTGAGGCGGCTGTTCGTGGACAAAGGGAGCT
TGCTGCCTATCTCTCCACAAAGATGGAAACACAAAAAATCTCGATTCAGGACGCAGATAACAACACTCGTCAGGTCGAAT
TACCGACGTCGGCACTGACATTATTGATGTCGATTTTAGGCGAGCTGGCTGTGGGTAACGCGGTTCAGGTTGTTCCTGTT
CATGCTGAATTAACGACACAGGAGGCAGCCAACATTCTGAATGTTTCACGCCCTCACATGGTGAAGCTTCTGGAGGATGG
GAAACTACCTTTTCATAAAACTGGTCGCCACCGCCGTGTGCTTTTCGCTGACCTGATGGACTATAAACACCAGAGGGATA
ATGAAAGCAGTAGAGCAATGCAGGCGTTGGCAGATCATAGCCAAGAGCTTGGATTGTACTAA

Structures

Toxin

• AlphaFold DB: A8AL02