Detailed information of TA system TA183910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 997854..998588
• Replicon: chromosome
• Accession: NZ_CP066089
• Organism: Citrobacter koseri strain FDAARGOS_1029

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A8AI82
• Locus tag: I6H86_RS04840
• Protein ID: WP_012132927.1
• Coordinates: 998283..998588 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A8AI81
• Locus tag: I6H86_RS04835
• Protein ID: WP_012132926.1
• Coordinates: 997854..998195 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H86_RS04810	993339..993962	-	624	WP_012132918.1	thioredoxin domain-containing protein	-
I6H86_RS04815	993959..995845	-	1887	WP_012132919.1	thioredoxin family protein	-
I6H86_RS04820	995894..996259	-	366	WP_012132921.1	hypothetical protein	-
I6H86_RS04825	996489..997409	+	921	WP_012132923.1	curved DNA-binding protein	-
I6H86_RS04830	997409..997714	+	306	WP_012132924.1	chaperone modulator CbpM	-
I6H86_RS04835	997854..998195	-	342	WP_012132926.1	HigA family addiction module antidote protein	Antitoxin
I6H86_RS04840	998283..998588	-	306	WP_012132927.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H86_RS04845	998817..999923	+	1107	WP_012132929.1	4Fe-4S binding protein	-
I6H86_RS04850	999957..1000733	-	777	WP_012132930.1	ABC transporter ATP-binding protein	-
I6H86_RS04855	1000733..1001503	-	771	WP_012132931.1	ABC transporter permease	-
I6H86_RS04860	1001500..1002507	-	1008	WP_012132932.1	ABC transporter substrate-binding protein	-
I6H86_RS04865	1002536..1003255	-	720	WP_012132933.1	aspartate/glutamate racemase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11972.70 Da
• Isoelectric Point: 8.3565

>T183910 WP_012132927.1 NZ_CP066089:c998588-998283 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKIPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Nucleotide

• Length: 306 bp

>T183910 NZ_CP087205:4410931-4411239 [Pseudomonas sp. B21-053]
ATGAGCCAAACTTTGACGACATTCGACATGGCAGCGCTGCTCGACAGTGATGAGGCCATCAGCGAATATCTCTCCCAGGT
ACTGGCAGAGGGCGACAACGAAGAGTTTCTCCGCGCTATCGGCTACGTGGTAAAAGCCCGTGGTATGACACAAATCGCCA
AAGATTCCGGGATGGGGCGCGAAAGCCTCTACAAGGCCTTCGCTCCGGGTGCAAAACCACGTTTTGATACGGTGCTAAAG
GTCATTCACGCCCTCGGAATTGATCTCTATGCGCAGCCAGGTCACAACCTCAATCATTCGAATCTCTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13062.91 Da
• Isoelectric Point: 5.1864

>AT183910 WP_012132926.1 NZ_CP066089:c998195-997854 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERQKQEKKVA

Nucleotide

• Length: 342 bp

>AT183910 NZ_CP087205:4410635-4410928 [Pseudomonas sp. B21-053]
ATGAACTATCTTATTCAACAAACACATACTTTCTCGGCATGGCACGTCTCAGTTCGTGACTTACGGGCAAAGTTCGCCAT
CGCTCGTCGCATTGATCGTGCCTCTGCGGGTAATCTAGGCGACATCAAGTCGCTGGGTGATGGTGTTTCCGAAATGCGCG
TGGACATTGGTGCAGGCTATCGAATCTACTTCACCATGCGAAACGGCGTGGTCATCGTATTATTGGCCGGCGGCGACAAA
TCCTCGCAAAGCTCTGATATCCGGCGTGCAAAAAAAATGGCAAAGGAGGTTTAA

Structures

Toxin

• AlphaFold DB: A8AI82

Antitoxin

• AlphaFold DB: A8AI81