Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3975636..3976138 Replicon chromosome
Accession NZ_CP066085
Organism Escherichia fergusonii strain FDAARGOS_1032

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I6H89_RS19150 Protein ID WP_000767829.1
Coordinates 3975636..3975890 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6H89_RS19155 Protein ID WP_001259255.1
Coordinates 3975887..3976138 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H89_RS19120 3970653..3970880 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
I6H89_RS19125 3970894..3971952 - 1059 WP_000492054.1 transport protein YeeE -
I6H89_RS19130 3972130..3973488 - 1359 WP_000019201.1 putrescine/proton symporter PlaP -
I6H89_RS19135 3973478..3973540 - 63 WP_010723108.1 membrane protein YoeI -
I6H89_RS19140 3973754..3974683 - 930 WP_000803362.1 LysR family transcriptional regulator -
I6H89_RS19145 3974729..3975553 - 825 WP_000754724.1 SDR family oxidoreductase -
I6H89_RS19150 3975636..3975890 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6H89_RS19155 3975887..3976138 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6H89_RS19160 3976422..3976472 + 51 WP_001364200.1 his operon leader peptide -
I6H89_RS19165 3976618..3977517 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
I6H89_RS19170 3977523..3978827 + 1305 WP_015953522.1 histidinol dehydrogenase -
I6H89_RS19175 3978824..3979894 + 1071 WP_000108922.1 histidinol-phosphate transaminase -
I6H89_RS19180 3979894..3980961 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T183899 WP_000767829.1 NZ_CP066085:c3975890-3975636 [Escherichia fergusonii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T183899 NZ_CP087204:c879240-878962 [Pseudomonas sp. B21-054]
ATGATAAGAAGCTTCAGATGCGCCGACACCCAAGCTCTTTTTGAAACCGGCAAAACCCGGCGCTGGGCAGCCGTCCTTTC
AGTGGCAGAACGAAAGCTGGGCATGCTCGATGCTGCGGTCGATCTCAACGACCTGCGCTCTCCTCCTGGAAACAGGTTGG
AGCAGCTGTATGGCGACAGGGAAGGCCAGCACAGCATCCGGGTTAACGGTCAGTACCGGATCTGTTTTGTCTGGGGCGCC
AACGGCCCCGAAAATGTAGAAATCGTAGATTATCACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT183899 WP_001259255.1 NZ_CP066085:c3976138-3975887 [Escherichia fergusonii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT183899 NZ_CP087204:c878953-878648 [Pseudomonas sp. B21-054]
ATGTTTAAGAATGGCATGAGACCCGTACACCCTGGAGAAATTCTCAAAGAAGAGTATCTGGAACCGCTTGGCCTTACCGC
GGCTGCGCTTGCGAGGGCTTTGCACGTTTCTGCCCCCACCGTAAATGACATTGTCTTGATGCGCCGTAGCATCAGCGCCG
ATGTTGCTTTGCGCCTGGCCGCCGCATTGGAAACGTCAGCGCAGTTTTGGCTGAATCTGCAGACTGCGTATGATTTACGT
ACAGCTGAGATTGCTAAAGGGGAACAGATTCACAGGGAAGTGGAAAAGGTTCCTCACTGCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References