Detailed information of TA system TA183889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2389954..2390633
• Replicon: chromosome
• Accession: NZ_CP066085
• Organism: Escherichia fergusonii strain FDAARGOS_1032

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: I6H89_RS11520
• Protein ID: WP_002431667.1
• Coordinates: 2389954..2390256 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6H89_RS11525
• Protein ID: WP_000806441.1
• Coordinates: 2390292..2390633 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H89_RS11495	2385330..2386982	+	1653	WP_000670595.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
I6H89_RS11500	2387020..2387523	-	504	WP_000667001.1	hypothetical protein	-
I6H89_RS11505	2387520..2388320	-	801	WP_000439795.1	hypothetical protein	-
I6H89_RS11510	2388344..2388823	-	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
I6H89_RS11515	2389027..2389821	-	795	WP_000365145.1	TraB/GumN family protein	-
I6H89_RS11520	2389954..2390256	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H89_RS11525	2390292..2390633	+	342	WP_000806441.1	HigA family addiction module antidote protein	Antitoxin
I6H89_RS11530	2390747..2393251	-	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
I6H89_RS11535	2393558..2394847	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T183889 WP_002431667.1 NZ_CP066085:2389954-2390256 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T183889 NZ_CP087201:4410996-4411346 [Pseudomonas sp. B21-059]
ATGAATGCTCTGTTTATTGAACTACCGGCATTTGCACGGTACCGAAAGGACTTCCTTGACGACGAGTTGTTCTGCGAACT
GCAGATCGAGTTGCTGCGCCACCCGCACGCAGGAGTTCTGATTCAGGGCACTGGCGGCTTGCGCAAGATGCGTTTCGTCG
ATGAGCGACGCAACAAGGGCAGGCGTGGCGGGCTGAGAGTGGTTTATTACTGGTGGTCCGGAGGGGCGCAGTTCTGGCTC
TTCACCCTGTTCGACAAGGAGCAGATGGACGACCTGCTACCGCAGCAGCGACAACAGCTCAAACAACTGCTGGAACGTGA
AATCGAGGCCAGAAGACATGACCAAACGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT183889 WP_000806441.1 NZ_CP066085:2390292-2390633 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT183889 NZ_CP087201:4411333-4411650 [Pseudomonas sp. B21-059]
ATGACCAAACGTGACATCTTTTCCGAACTGGTAGAAGGCATCGACGCCCTCACCCAGGAGCGCCAGGGCAAGATCACCCT
GCGCAGCCATAAAGTGAAGCTGGACAAACTGCCCCCCATCACTGCCACCGAGGTGATGAACATCCGCCAGCAACTCAACC
TCTCGCGTTCAGTATTCGCCATGTACCTGCGCACCAACACCCGTACCCTGGAAAACTGGGAACAAGGCCGCGCCACACCC
AACGCCCAGGCCACGACCTTGATGCGCCTGGTGGAACGCTACCCGGACACAGTGGAGCGCCTCGCCTCGCTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8