Detailed information of TA system TA183885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1735355..1736154
• Replicon: chromosome
• Accession: NZ_CP066085
• Organism: Escherichia fergusonii strain FDAARGOS_1032

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7LMV3
• Locus tag: I6H89_RS08280
• Protein ID: WP_000347277.1
• Coordinates: 1735355..1735819 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: B7LMV4
• Locus tag: I6H89_RS08285
• Protein ID: WP_015953962.1
• Coordinates: 1735819..1736154 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H89_RS08250	1730357..1730791	-	435	WP_000948832.1	PTS sugar transporter subunit IIA	-
I6H89_RS08255	1730809..1731687	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
I6H89_RS08260	1731677..1732456	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
I6H89_RS08265	1732467..1732940	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6H89_RS08270	1732963..1734243	-	1281	WP_000681964.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6H89_RS08275	1734491..1735300	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6H89_RS08280	1735355..1735819	-	465	WP_000347277.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6H89_RS08285	1735819..1736154	-	336	WP_015953962.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6H89_RS08290	1736303..1737874	-	1572	WP_000247251.1	galactarate dehydratase	-
I6H89_RS08295	1738345..1739115	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
I6H89_RS08300	1739140..1740030	+	891	WP_000178101.1	2-hydroxy-3-oxopropionate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T183885 WP_000347277.1 NZ_CP066085:c1735819-1735355 [Escherichia fergusonii]
MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T183885 NZ_CP087201:c723479-723177 [Pseudomonas sp. B21-059]
ATGTTCGTCTTTGAGCAGACTCAGGCGTTCACCCACTGGCTGCTACGCCTAAAAGATCCCCAGGGAAAAACCCGCGTGCT
GGCCCGGATCAGGGCGGCGCAACTGGGCCGTTTCGGCGACTGCGAACCGGTGGGCGAAGGTGTCTATGAAATGCGCATCC
ATCGCGGCCCCGGCTATCGGGTGTACTTCTCGCGCCGGGACACCGTGATCTACCTGTTGCTGATCGGCGGTGACAAGGGC
ACCCAGAGACGCGATATCCAACGCGCCAGGCAACTGGCCCATGACCTCAAGAGCGAGGGCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12281.89 Da
• Isoelectric Point: 4.4128

>AT183885 WP_015953962.1 NZ_CP066085:c1736154-1735819 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT183885 NZ_CP087201:c723173-722883 [Pseudomonas sp. B21-059]
ATGCGTACCCCAGTGACCCGCTTCGACGCGGCAGAGTACCTCAAGACCCCGGAAGACATGGTGGCCTACCTCGACGCCTG
CTTCGAGGAAGATGCCGGCGACGGCAAGCTGATCCGCGCCGCCCTCAACGACATCGCCCGCGCCCGGGGCATGACCCAGG
TGGCCCGCGACACCGGCCTGGGTCGCGAGAGCCTGTACAAGGCCCTGGGCAGCCAGGGCAATCCCGAGTTCGCGACTATC
ATCAAGGTCATGAAGGCACTGGGACTCAAGCTCCACGTCAGCCCGGCCTGA

Structures

Toxin

• AlphaFold DB: B7LMV3

Antitoxin

• AlphaFold DB: B7LMV4