Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 64854..65548 Replicon chromosome
Accession NZ_CP066085
Organism Escherichia fergusonii strain FDAARGOS_1032

Toxin (Protein)


Gene name yafO Uniprot ID B7LNG9
Locus tag I6H89_RS00445 Protein ID WP_001263502.1
Coordinates 64854..65252 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag I6H89_RS00450 Protein ID WP_000554758.1
Coordinates 65255..65548 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H89_RS00415 59982..61439 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
I6H89_RS00420 61487..61729 + 243 WP_001029361.1 hypothetical protein -
I6H89_RS00425 61738..62262 - 525 WP_024256511.1 hydrolase -
I6H89_RS00430 62324..62938 - 615 WP_000602110.1 peptide chain release factor H -
I6H89_RS00435 62935..64074 - 1140 WP_000521571.1 RNA ligase RtcB family protein -
I6H89_RS00440 64392..64844 - 453 WP_001059849.1 GNAT family N-acetyltransferase -
I6H89_RS00445 64854..65252 - 399 WP_001263502.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
I6H89_RS00450 65255..65548 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
I6H89_RS00455 65600..66655 - 1056 WP_001226166.1 DNA polymerase IV -
I6H89_RS00460 66726..67511 - 786 WP_000207582.1 putative lateral flagellar export/assembly protein LafU -
I6H89_RS00465 67492..69195 + 1704 Protein_91 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15486.90 Da        Isoelectric Point: 8.9401

>T183872 WP_001263502.1 NZ_CP066085:c65252-64854 [Escherichia fergusonii]
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T183872 NZ_CP087198:c757733-757443 [Pseudomonas sp. B21-010]
ATGACTGAAGAATTCACACGTTTCGATGCAGCCACTTACCTCAAGACGCCTGAGGAAATGGCCGCTTATCTGGATGCCTG
TTTTGAAGAGGATGCGGGTGACGGCGTACTGGTTCGAGCAGCTCTTAACGATATTGCCAGGGCAAAGGGCATGACGCAGA
TTGCTCGTGATGCAGGCCTGGGGCGAGAGAGTCTTTACAAAGCACTGGGAAGTACGGGCAATCCGGAGTTCACAACTATC
ATGAAAGTCATGAAAGCTTTGGGCTTGAGGTTACATGCGACGGCCATTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT183872 WP_000554758.1 NZ_CP066085:c65548-65255 [Escherichia fergusonii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT183872 NZ_CP087198:c758039-757737 [Pseudomonas sp. B21-010]
ATGATCGAATTTGAACAGTCCCGAGTATTCTCAAGCTGGCTTAACGGGTTGAAGGACATTGCTGGGAAAGCTCGCATCAT
TGCCAGGCTCCGATCGGCAGAGCATGGAAATTTTGGTGATTGCGAACCGGTGGGTAACGCAGTGTATGAGATGCGTATTC
ACTCGGGTCCGGGCTACAGAGTGTACTTCTCCCGTCAAGGCTCCGTGCTTTATTTGCTGCTTGTGGGAGGTGACAAATCG
ACTCAGAAACGGGATATCGCACGCGCTGTTCAAATGGCGCAAACCATGGGTAATGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B7LNG9

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References