Detailed information of TA system TA183841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 701007..701597
• Replicon: chromosome
• Accession: NZ_CP066058
• Organism: Alcaligenes faecalis strain FDAARGOS_1024

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A2N9E1
• Locus tag: I6H81_RS03250
• Protein ID: WP_035268575.1
• Coordinates: 701007..701303 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: I6H81_RS03255
• Protein ID: WP_042484646.1
• Coordinates: 701310..701597 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H81_RS03235	696799..697695	-	897	WP_042484636.1	MurR/RpiR family transcriptional regulator	-
I6H81_RS03240	697852..698130	-	279	WP_042484640.1	acylphosphatase	-
I6H81_RS03245	698360..700873	+	2514	WP_042484643.1	EAL domain-containing protein	-
I6H81_RS03250	701007..701303	+	297	WP_035268575.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H81_RS03255	701310..701597	+	288	WP_042484646.1	HigA family addiction module antidote protein	Antitoxin
I6H81_RS03260	701708..703075	-	1368	WP_042484649.1	DNA recombination protein RmuC	-
I6H81_RS03265	703354..704364	+	1011	WP_042484652.1	acyltransferase family protein	-
I6H81_RS03270	704563..705810	-	1248	WP_042484654.1	D-amino acid dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11108.48 Da
• Isoelectric Point: 9.3459

>T183841 WP_035268575.1 NZ_CP066058:701007-701303 [Alcaligenes faecalis]
MIKSFRHKGLQAYFETGSKSGIQAKHAVRLQIQLTALHSATQAEDMNAPGWKLHKLSGKNPKGQSVQDHYTISVSGNWRL
TFYFEGEDAVLVDYQDYH

Nucleotide

• Length: 297 bp

>T183841 NZ_CP087190:304918-305109 [Pseudomonas sp. B21-023]
ATGAACAGCCGTGAAATGATTGCTGTGATCGAGGCGGATGGCTGGTATCTGGTGCGCTCCAAAGGCAGCCACCATCATTT
CAAGCATCCTGAAAAAGCCGGGCTGGTCACCATCCCGCATCCCAAGAAAGACCTGCTGCCCGCGACTGCGGCCAGCATTC
TGCGCCAGGCACGCATCAGCTACGCCAGCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10430.96 Da
• Isoelectric Point: 9.8929

>AT183841 WP_042484646.1 NZ_CP066058:701310-701597 [Alcaligenes faecalis]
IMRMHNPSHPGPVLREYLGELSVTEAASRLGVTRAALSRILNGSAGISADMALRLRDALGTSAEMWINMQSHYDLWQAEQ
KPRPKVVSFHGPAHA

Nucleotide

• Length: 288 bp

>AT183841 NZ_CP087190:305126-305539 [Pseudomonas sp. B21-023]
ATGTTGTTCCCCATCGCGATCCTCCCCGGTGACGACCAGCACGCCTGGGGCGTGGAAGTCCCGGATATCCCCGGCTGCTT
TTCGGCGGGCGAAGACCTCGACGACGCTATCGCCATGGCGCGTGAAGCGATCGAAGGCCACCTCGAACTGCTGGCCCAGG
ACCAGCAGGAAATCCCCAGGGCCAGCAAGGTCGGCGTGCATGCGGCCAATCCCGCCTATGCCGGCTGCACCTGGGCATTG
ATCGATATCGACATCACCCGCTACCTGGGCAAGGCCGAGAAGCTCAACATCACCTTGCCGGCGTACCTGCTCACCCGCAT
CGACAGCTACGTACAGAACCACCCGGAGCACAAGAGCCGTTCGGGCTTCCTGGCCGAAGCGGCGCTCAGGATCCTGCAGG
GTGACGGCAAATGA

Structures

Toxin

• AlphaFold DB: A0A0A2N9E1