Detailed information of TA system TA183836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 14824..15414
• Replicon: plasmid unnamed2
• Accession: NZ_CP066052
• Organism: Brucella anthropi strain FDAARGOS_1039

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A6X893
• Locus tag: I6H96_RS15150
• Protein ID: WP_011983054.1
• Coordinates: 14824..15105 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6H96_RS15155
• Protein ID: WP_011983055.1
• Coordinates: 15118..15414 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H96_RS15125	10436..11479	+	1044	WP_011983049.1	plasmid partitioning protein RepB	-
I6H96_RS15130	11634..12857	+	1224	WP_011983050.1	replication initiation protein RepC	-
I6H96_RS15135	12959..13384	+	426	WP_011983051.1	DUF5086 domain-containing protein	-
I6H96_RS15140	13399..13779	+	381	WP_011983052.1	DUF2195 family protein	-
I6H96_RS15145	14033..14662	-	630	WP_011983053.1	SH3 domain-containing protein	-
I6H96_RS15150	14824..15105	+	282	WP_011983054.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H96_RS15155	15118..15414	+	297	WP_011983055.1	HigA family addiction module antidote protein	Antitoxin
I6H96_RS15160	15793..16305	+	513	WP_011983057.1	hypothetical protein	-
I6H96_RS15165	17677..18315	+	639	WP_011983059.1	hypothetical protein	-
I6H96_RS15170	18410..18727	+	318	WP_040128186.1	hypothetical protein	-
I6H96_RS15175	18743..19758	-	1016	Protein_19	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61780	61780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10479.00 Da
• Isoelectric Point: 9.0323

>T183836 WP_011983054.1 NZ_CP066052:14824-15105 [Brucella anthropi]
MIRSYKDARTEAVAAGKAPKGFPADLIRTTVRKLTMIDSAVILDDLRSPPGNRLEALKGDRKGQHSIRINDQWRICFVWT
DAGAEQIEIVDYH

Nucleotide

• Length: 282 bp

>T183836 NZ_CP087189:1011502-1011780 [Pseudomonas protegens]
ATGAGACAGCTGAAATGGACGAGCAAGGCCCTATCCGATCTGGCTCGGCTTTTTGAGTTTCTCGCCCAGGCCAATCGTTC
TACTGCTGCTCGGACCGTGCAATCGCTGGTGCAGGCTCCAGATGCTCTTCTATCTAACCCTCGATTGGGAGAGCAGTTGG
AGGAATTCAAGCCGAGGGATGTTCGACGATTGCTGGTGGGGCATTACGAGATGCGTTATGAGATTCAAGGCATGGCCTTG
TCCATCCTGCGTATTTGGCATGTCCGGGAAGATCGTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10929.70 Da
• Isoelectric Point: 7.2026

>AT183836 WP_011983055.1 NZ_CP066052:15118-15414 [Brucella anthropi]
MTSKLPPVHPGEILREEYLIPLGMSAGALAKNLNVPRTRIERLATEQTAVTPDTALRLAKFFRTTPEFWMNLQSGYDLKT
EAVAIQDQLLAIHELKAA

Nucleotide

• Length: 297 bp

>AT183836 NZ_CP087189:1011242-1011505 [Pseudomonas protegens]
ATGCCCGCGACAAAAGTACTGACCGCTCACATTCCCTTGGACCTGGCTGACAAGGTCGATCAACTTGCTTCTCGCCTGGA
ACGCTCCCGGGCCTGGGTCGTGAAGCAGGCGTTGGTGGCCTGGGTTGACCAGGAAGAAGAGCGCCGTCGACTGACGCTTG
AGGCCTTGGCAGATGTCGATGCTGGAAGGGTTGTCGATCATCAGTCAGTGTTGGCTTGGGCGCAGAGTCTGTCTGGCGAC
AACCCTCTGCCCTTACCAAAATGA

Structures

Toxin

• AlphaFold DB: A6X893