Detailed information of TA system TA183818

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58422..58686
• Replicon: plasmid unnamed2
• Accession: NZ_CP066034
• Organism: Escherichia coli strain FDAARGOS_1059

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: I6I16_RS26865
• Protein ID: WP_001303307.1
• Coordinates: 58534..58686 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	58422..58484 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I16_RS26850	53661..55952	-	2292	WP_001289271.1	hypothetical protein	-
I6I16_RS26855	55945..57015	-	1071	WP_063097584.1	IncI1-type conjugal transfer protein TrbB	-
I6I16_RS26860	57034..58242	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	58422..58484	-	63	NuclAT_0	-	Antitoxin
-	58422..58484	-	63	NuclAT_0	-	Antitoxin
-	58422..58484	-	63	NuclAT_0	-	Antitoxin
-	58422..58484	-	63	NuclAT_0	-	Antitoxin
I6I16_RS26865	58534..58686	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
I6I16_RS26870	58989..59770	-	782	Protein_73	IS21-like element IS100kyp family helper ATPase IstB	-
I6I16_RS26875	59767..60789	-	1023	WP_000255946.1	IS21-like element IS100 family transposase	-
I6I16_RS26880	61626..61802	-	177	WP_001054898.1	hypothetical protein	-
I6I16_RS26885	62145..62354	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
I6I16_RS26890	62426..63088	-	663	WP_000653333.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88626	88626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T183818 WP_001303307.1 NZ_CP066034:58534-58686 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T183818 NZ_CP087187:c326453-326055 [Pseudomonas atacamensis]
ATGATCAAGTACATGCTGGATACAAATATCTGCATCTTCACCATCAAGAACAAACCACAAATTGTCCGGGAAGCCTTCAA
TCGACATTCCGGGCAGTTGTGCATCAGCGCTATCACGCTTATGGAGCTTATTTACGGTGCTGAAAAGTCGGCGGCCCCGG
AGAAAAATCTGGCAATTGTTGAAGGATTTGTCGCGCGACTGGATGTCTTGCCTTTCGATAACGACGCAGCCGCACAGGCC
GGGATGGTTCGATCTGAACTCGCGAAGGCTGGAACGCCGATCGGACCATACGACCAAATGATCGCTGGCCACGCACGCTC
ACTTGGACTCATTGTAGTGACGAACAACGTACGGGAGTTTCAACGCGTTTCCGGTTTGCGAATCGAGGACTGGGTTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT183818 NZ_CP066034:c58484-58422 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin