Detailed information of TA system TA183792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1066357..1066959
• Replicon: chromosome
• Accession: NZ_CP066032
• Organism: Escherichia coli strain FDAARGOS_1059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6I16_RS05065
• Protein ID: WP_000897305.1
• Coordinates: 1066648..1066959 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I16_RS05060
• Protein ID: WP_000356397.1
• Coordinates: 1066357..1066647 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I16_RS05035	1061923..1062558	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6I16_RS05040	1062555..1063484	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I6I16_RS05045	1063883..1064863	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
I6I16_RS05050	1065092..1065334	-	243	WP_001086388.1	hypothetical protein	-
I6I16_RS05055	1065553..1065771	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I6I16_RS05060	1066357..1066647	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6I16_RS05065	1066648..1066959	-	312	WP_000897305.1	hypothetical protein	Toxin
I6I16_RS05070	1067188..1068096	+	909	WP_001162704.1	alpha/beta hydrolase	-
I6I16_RS05075	1068160..1069101	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
I6I16_RS05080	1069146..1069583	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6I16_RS05085	1069580..1070452	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6I16_RS05090	1070446..1071045	-	600	WP_001295269.1	glucose-1-phosphatase	-
I6I16_RS05095	1071144..1071929	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1063883..1064863	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T183792 WP_000897305.1 NZ_CP066032:c1066959-1066648 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T183792 NZ_CP087182:4616645-4616995 [Pseudomonas protegens]
ATGAATGCTCTGTTTATTGAACTACCGGCATTTGCGCGATACCGAAAAGGCTTCCTTGACGACGAGTTGTTCTGCGAACT
GCAGATCGAGTTGCTGCGTCACCCGCACGCGGGTGTTCTGATTCAGGGCACTGGCGGCTTGCGCAAGATGCGTTTCATCG
ATGAGCGACGCAACAAGGGCAGGCGTGGCGGGCTGAGGGTGATTTATTACTGGTGGTCCGGAGGAGCACAGTTCTGGCTC
TTCACCCTGTTCGACAAGGAGCAGATGGACGACCTGCTACCGCAGCAGCGACAACAGCTCAAACAACTGCTGGAACGTGA
AATCGAGGCCAGAAGACATGACCAAACGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT183792 WP_000356397.1 NZ_CP066032:c1066647-1066357 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT183792 NZ_CP087182:4616982-4617299 [Pseudomonas protegens]
ATGACCAAACGTGACATCTTTTCCGAACTGGTTGAAGGCATCGACGCCCTCACCCAGGAACGCCAGGGCAAGATCACCCT
GCGCAGCCATAAAGTGAAACTCGACAAGCTGCCCCCCATCACCGCCGCCGAGGTGATGAACATCCGCCAGCAACTCAACC
TCTCGCGCTCGGTATTCGCCATGTACCTGCGCACCAACACCCGCACCCTGGAGAACTGGGAACAGGGCCGCGCCATACCC
AACGCCCAGGCCACGACCTTGATACGCCTGGTGGAACGCTACCCCGATACAGTGGAGCGCCTCGCCTCGCTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7