Detailed information of TA system TA183752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1688544..1689155
• Replicon: chromosome
• Accession: NZ_CP065999
• Organism: Kingella denitrificans strain FDAARGOS_1060

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F0EYD6
• Locus tag: I6I17_RS08565
• Protein ID: WP_003782203.1
• Coordinates: 1688874..1689155 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I17_RS08560
• Protein ID: WP_003782201.1
• Coordinates: 1688544..1688861 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I17_RS08520	1684591..1685403	-	813	WP_003782186.1	hypothetical protein	-
I6I17_RS08525	1685415..1685636	-	222	WP_003782188.1	hypothetical protein	-
I6I17_RS08530	1685779..1686399	+	621	WP_115324820.1	helix-turn-helix domain-containing protein	-
I6I17_RS08535	1686519..1686818	+	300	WP_003782192.1	type II toxin-antitoxin system MqsR family toxin	-
I6I17_RS08540	1686820..1687224	+	405	WP_003782194.1	type II toxin-antitoxin system MqsA family antitoxin	-
I6I17_RS08545	1687350..1687718	+	369	WP_003782197.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6I17_RS08550	1687715..1688029	+	315	WP_003782198.1	DNA-binding transcriptional regulator	-
I6I17_RS08555	1688094..1688492	-	399	WP_003782200.1	type II toxin-antitoxin system HicB family antitoxin	-
I6I17_RS08560	1688544..1688861	-	318	WP_003782201.1	HigA family addiction module antidote protein	Antitoxin
I6I17_RS08565	1688874..1689155	-	282	WP_003782203.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I17_RS08570	1689869..1690327	-	459	WP_003782207.1	hypothetical protein	-
I6I17_RS08575	1690560..1690949	+	390	WP_040564241.1	hypothetical protein	-
I6I17_RS08580	1690946..1691635	+	690	WP_003782210.1	hypothetical protein	-
I6I17_RS08585	1691892..1692155	+	264	WP_003782212.1	hypothetical protein	-
I6I17_RS08590	1692201..1692644	+	444	WP_003782213.1	virulence-associated protein VapD	-
I6I17_RS08595	1692977..1693864	+	888	WP_003782214.1	antA/AntB antirepressor family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (16-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10976.54 Da
• Isoelectric Point: 7.7781

>T183752 WP_003782203.1 NZ_CP065999:c1689155-1688874 [Kingella denitrificans]
MITDFYCKDTQALWEGKRVKRFANIERVALRKLQQIHAAADLNFLRIPPANRLEALKGDREGQYSICVNDQWRICFIWEN
GHASRVEIVDYHD

Nucleotide

• Length: 282 bp

>T183752 NZ_CP087174:984830-985129 [Pseudomonas donghuensis]
ATGAGTTTGTCACTTACTACGTTTGATATGGCCGCGTTGCTCGATAGCGATGAGACAATCAGCGAGTACCTTTCTCAAGT
GCTGGCGGATGGTGATCATGACGAACTGATTCGCGCCCTCGGCTATATCGCCAAGGCTCGCGGCATGAGCCAGATCGCAA
CTGCCAGTGGATTGGGGCGCGAGAGCCTGTACAAGGCGTTGACCCCCGGTGCGAAGCCTCGTTTTGACACTGTACTGAAG
GTCATCCGGGCGCTAGGGATTGATCTTCTGGCCCACCCTCATGCTTCGTCGAGACTCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11482.31 Da
• Isoelectric Point: 8.6250

>AT183752 WP_003782201.1 NZ_CP065999:c1688861-1688544 [Kingella denitrificans]
MNKREIPLIHPGEILLEDWLKPLGISQYALAKAIDVPPRRINEIILGKRGISADTALRLGAFFNTDAQSWLNLQSHYDAE
TARIALAPTLAHIVPFRAAGTAASV

Nucleotide

• Length: 318 bp

>AT183752 NZ_CP087174:984535-984828 [Pseudomonas donghuensis]
TTGAAATACAGTGTTTTGCAAACGCCCAGATTCACCTCTTGGTTGATTTCCGTACGCGACATGCGTGCAAGGGTGGCAAT
CGCACGGCGAATAGAGCGTGTAACGGCGGGAAATCTGGGAGATGTGAAGTCCGTCGGTGGTCAGGTCTGCGAGATGCGCA
TCGATGTGGGGGCGGGTTATCGGGTGTATTTCACGGCTCGGGACGGTGTAGTCATTGTTTTGTTGGCAGGTGGTGACAAG
TCATCGCAATCTGTTGATATCCAACTGGCCAGGAAGCTGGCCAAGGAAATTTGA

Structures

Toxin

• AlphaFold DB: F0EYD6