Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 2339617..2340187 Replicon chromosome
Accession NZ_CP065989
Organism Brevibacterium casei strain FDAARGOS_990

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag I6H47_RS10255 Protein ID WP_198498449.1
Coordinates 2339617..2339898 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6H47_RS10260 Protein ID WP_198498450.1
Coordinates 2339885..2340187 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H47_RS10235 2335113..2335571 + 459 WP_198498445.1 NUDIX domain-containing protein -
I6H47_RS10240 2336170..2337213 - 1044 WP_198498446.1 alcohol dehydrogenase catalytic domain-containing protein -
I6H47_RS10245 2337248..2338492 - 1245 WP_198498447.1 MFS transporter -
I6H47_RS10250 2338629..2339510 + 882 WP_198498448.1 helix-turn-helix transcriptional regulator -
I6H47_RS10255 2339617..2339898 + 282 WP_198498449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6H47_RS10260 2339885..2340187 + 303 WP_198498450.1 HigA family addiction module antidote protein Antitoxin
I6H47_RS10265 2340187..2340645 + 459 WP_198498451.1 hypothetical protein -
I6H47_RS10270 2341539..2341964 + 426 WP_198498452.1 DUF1801 domain-containing protein -
I6H47_RS10275 2342188..2343336 + 1149 WP_198498453.1 hypothetical protein -
I6H47_RS10280 2343362..2343631 - 270 WP_198498454.1 DUF3263 domain-containing protein -
I6H47_RS10285 2343765..2344181 - 417 WP_198498455.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2331272..2344004 12732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(5-97)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10922.44 Da        Isoelectric Point: 8.0756

>T183742 WP_198498449.1 NZ_CP065989:2339617-2339898 [Brevibacterium casei]
VIRPFGDKETERLWHRERVRSIDPRILRVALRKLRQLGSAEALEDLRVPPGNRLEALKGDRAGQHSIRINDQWRICFVWT
DAGPEEVEIVDYH

Download         Length: 282 bp

>T183742 NZ_CP087171:c872323-872072 [Pseudomonas atacamensis]
ATGTCAAAGACTGAAAAACTTCTCGCCAAGCTAATCAACGGGCACAGCGCATTCACTTGGTCCGAGCTTGTCACTCTGTT
AGGTCGTCTTGGTTACTCCCAACTGGAAGGATCCGGTAGCAGGATCAAATTCGACAATGGGGATCCGAACGCATTGATCA
ACCTGCACAAGCCCCATCCCGGAAATGAAATCAAAACATACGCAAGACGCCAGATTATCGAGCAACTGAAAAAAGGAGGC
CTGATTCAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11064.59 Da        Isoelectric Point: 4.8255

>AT183742 WP_198498450.1 NZ_CP065989:2339885-2340187 [Brevibacterium casei]
MTTTDKIEPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRGITADTALRLARYFGTSAEFWINLQSHYEL
DRAEELAGEQIASITPLSVA

Download         Length: 303 bp

>AT183742 NZ_CP087171:c872075-871734 [Pseudomonas atacamensis]
ATGAGTTCCCAGCTGCACTACAAAGGCTATGTTGGCTCTATCGAGGCGAGCACGGAAGATAAGTGCCTGTTCGGAAAACT
CCTTTTCATCAGGGCGCTGGTTAATTATGAAGGACAAACGGTGGCTGAACTGGAAGCAGCCTTTCGCGAGGCGGTAGATG
ACTATTTACAAACCTGTGAGCAGCTCGGGCAGAAGCCTGAAATCCCATGCAAAGGATCGTTCAATGTTCGCATCGGTCAC
GATCTGCATTTAGCAGCGGCTCTGGCGGCAACCCGTCAAAATGTGTCGTTGAATGATCTGACACGTCAAGCACTGAACGA
ATACCTCCAACACCACGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References