Detailed information of TA system TA183732

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60151..60577
• Replicon: plasmid unnamed3
• Accession: NZ_CP065971
• Organism: Escherichia coli strain FDAARGOS_1058

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6I15_RS25635
• Protein ID: WP_001312861.1
• Coordinates: 60151..60309 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60353..60577 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I15_RS25600	55205..55420	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
I6I15_RS25605	55556..56203	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
I6I15_RS25610	56394..56777	-	384	WP_001063020.1	relaxosome protein TraM	-
I6I15_RS25615	57117..57719	+	603	WP_000243707.1	transglycosylase SLT domain-containing protein	-
I6I15_RS25620	58015..58836	-	822	Protein_63	DUF945 domain-containing protein	-
I6I15_RS25625	58956..59243	-	288	WP_000107542.1	hypothetical protein	-
I6I15_RS25630	59268..59474	-	207	WP_032192990.1	hypothetical protein	-
I6I15_RS25635	60151..60309	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	60353..60577	-	225	NuclAT_0	-	Antitoxin
-	60353..60577	-	225	NuclAT_0	-	Antitoxin
-	60353..60577	-	225	NuclAT_0	-	Antitoxin
-	60353..60577	-	225	NuclAT_0	-	Antitoxin
I6I15_RS25640	60389..60577	+	189	WP_001299721.1	hypothetical protein	-
I6I15_RS25645	60589..61308	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
I6I15_RS25650	61305..61739	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6I15_RS25655	61794..63752	-	1959	WP_063121799.1	ParB/RepB/Spo0J family partition protein	-
I6I15_RS25660	63818..64051	-	234	WP_000005990.1	DUF905 family protein	-
I6I15_RS25665	64114..64653	-	540	WP_000290838.1	single-stranded DNA-binding protein	-
I6I15_RS25670	64679..64885	-	207	WP_000275853.1	hypothetical protein	-
I6I15_RS25675	65296..65520	-	225	WP_063123567.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / cnf1 / cdtA / cdtB / cdtC / iutA / iucD / iucC / iucB / iucA	1..128137	128137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T183732 WP_001312861.1 NZ_CP065971:c60309-60151 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T183732 NZ_CP087169:4352609-4352911 [Pseudomonas sp. B21-047]
ATGAAGAAAATCGAATCCAGCAGTTTCAGGCATTGGGTGCATGGACTCCGGGACGACACGGCGCGTATCCGGATTATTGC
TCGTATCAACCGCTTGATGGAGGGCCTGCCTGGCGACGTTTCAGCAGTTGGCCATGGTGTAAGTGAATTGAGAATTCACT
ATGGGCCGGGCTACCGCGTGTACTTTCACCAATCAGGCGATACGTTGGTAATTCTGCTCTGCGGCGGGCACAAAAGCAGC
CAACGGCGGGACATCAAAGCGGCTCACGAAATCCTGCGCTCCTGGAGGATGCAACATGACTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT183732 NZ_CP065971:c60577-60353 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGTGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin