Detailed information of TA system TA183728

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21221..21475
• Replicon: plasmid unnamed3
• Accession: NZ_CP065971
• Organism: Escherichia coli strain FDAARGOS_1058

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: I6I15_RS25415
• Protein ID: WP_001362723.1
• Coordinates: 21221..21427 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	21414..21475 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I15_RS25375	16394..16819	-	426	Protein_14	transposase	-
I6I15_RS25380	16807..17831	+	1025	Protein_15	IS3 family transposase	-
I6I15_RS25385	17980..18267	-	288	WP_000865085.1	helix-turn-helix transcriptional regulator	-
I6I15_RS25390	18267..18578	-	312	WP_000483535.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6I15_RS25395	18684..18824	-	141	WP_001315588.1	hypothetical protein	-
I6I15_RS25400	19523..20380	-	858	WP_000130977.1	incFII family plasmid replication initiator RepA	-
I6I15_RS25405	20373..20447	-	75	WP_001365705.1	RepA leader peptide Tap	-
I6I15_RS25410	20689..20937	-	249	WP_000083838.1	replication regulatory protein RepA	-
I6I15_RS25415	21221..21427	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	21414..21475	+	62	NuclAT_1	-	Antitoxin
-	21414..21475	+	62	NuclAT_1	-	Antitoxin
-	21414..21475	+	62	NuclAT_1	-	Antitoxin
-	21414..21475	+	62	NuclAT_1	-	Antitoxin
I6I15_RS25420	21650..22033	-	384	WP_001109067.1	hypothetical protein	-
I6I15_RS25425	22136..22597	-	462	WP_000760085.1	thermonuclease family protein	-
I6I15_RS25430	23340..23543	-	204	WP_001327131.1	hypothetical protein	-
I6I15_RS25435	23698..24255	-	558	WP_000840474.1	fertility inhibition protein FinO	-
I6I15_RS25440	24358..25218	-	861	WP_000704525.1	alpha/beta hydrolase	-
I6I15_RS25445	25277..26023	-	747	WP_000205769.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / cnf1 / cdtA / cdtB / cdtC / iutA / iucD / iucC / iucB / iucA	1..128137	128137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T183728 WP_001362723.1 NZ_CP065971:c21427-21221 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T183728 NZ_CP087169:304796-305086 [Pseudomonas sp. B21-047]
ATGCACACACTAATTCAAACCGATAGGTACAGAAGGTGGTTCTCTGCCCTGCGTGATGATCGAGCGCAAGCTCGTATCAA
TATTCGATTGCGTCGAATCGAGCTGGGAGTATTGGGTGATTGTAAGCCGGTAGGTGAGGGAGTATCCGAGCTTCGGATTG
ACTACGGTCCAGGTTATCGGGTGTATTTCGTTCAGCGGGGCTATGAGGTAATCATTCTCTTGGCGGGAGGTGACAAGGCT
AGCCAGGCCGCGGACATCAAGTCAGCACTCAAGCTGGCACGCAATCTTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT183728 NZ_CP065971:21414-21475 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin