Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2854890..2855392 Replicon chromosome
Accession NZ_CP065970
Organism Escherichia coli strain FDAARGOS_1058

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag I6I15_RS14100 Protein ID WP_000767820.1
Coordinates 2855138..2855392 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6I15_RS14095 Protein ID WP_001259255.1
Coordinates 2854890..2855141 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I15_RS14070 2850067..2851134 - 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6I15_RS14075 2851134..2852204 - 1071 WP_000108952.1 histidinol-phosphate transaminase -
I6I15_RS14080 2852201..2853505 - 1305 WP_039025710.1 histidinol dehydrogenase -
I6I15_RS14085 2853511..2854410 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6I15_RS14090 2854556..2854606 - 51 WP_001364200.1 his operon leader peptide -
I6I15_RS14095 2854890..2855141 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6I15_RS14100 2855138..2855392 + 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6I15_RS14105 2855475..2856299 + 825 WP_000754756.1 SDR family oxidoreductase -
I6I15_RS14110 2856345..2857274 + 930 WP_000803351.1 LysR family transcriptional regulator -
I6I15_RS14115 2857489..2857551 + 63 WP_010723108.1 membrane protein YoeI -
I6I15_RS14120 2857541..2858899 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I6I15_RS14125 2859078..2860136 + 1059 WP_000492324.1 transport protein YeeE -
I6I15_RS14130 2860150..2860377 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T183714 WP_000767820.1 NZ_CP065970:2855138-2855392 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T183714 NZ_CP087167:c285320-285006 [Pseudomonas atacamensis]
ATGATTTTTATTGAGACTTCAGTCTTCACCCGCCGAGTAAGAGAGTTACTGGACGACGATGCCTACGCCACCTTTCAAAA
GCAGTTGGTCGCAAACCCCTCCATTGGCGATGTAATAGAAGGAACCGGCGGGGTGCGTAAAGCACGAGTCGCCTCCAAAG
GCCATGGCAAACGGGGTGGCGCACGGGTTATCTACTACCACTTCGTTTCAGCATCACAGATCGCGTTGTTGATGATTTAT
CCAAAAAACGAGCAGCAGGATCTTACCCATGACGAACGGAAAGCACTGAAGGCCATGATTGGAAACTGGAGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT183714 WP_001259255.1 NZ_CP065970:2854890-2855141 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT183714 NZ_CP087167:c285003-284716 [Pseudomonas atacamensis]
ATGAGCAAGTTTTTTGATGAGCTGATGGAAAGCGTGCAGGAAATGGATGAGATTCTTCGGGGTGAAAAAAAGCCGTCCCG
CGAATTCTATGTGGATGCATTACAGGTTAAACAGATTCGCAAAGCGACGGGAATGACGCAGGCAAAGTTCGCAGCCTTGA
TCGATGTCCAATTGGGAACGTTACGCAATTGGGAGCAAGGTCGTCGTCAGCCGACTGGGCCGGCCAAAGCACTACTCAAG
GCCATTCATAACGATCCTGTCCATGTGCTGAGTGCACTGGCTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References